利用下一代蛋白质微阵列推进转化研究。
Advancing translational research with next-generation protein microarrays.
作者信息
Yu Xiaobo, Petritis Brianne, LaBaer Joshua
机构信息
State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (The PHOENIX Center, Beijing), Beijing, P. R. China.
The Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ, USA.
出版信息
Proteomics. 2016 Apr;16(8):1238-50. doi: 10.1002/pmic.201500374. Epub 2016 Mar 31.
Abstract
Protein microarrays are a high-throughput technology used increasingly in translational research, seeking to apply basic science findings to enhance human health. In addition to assessing protein levels, posttranslational modifications, and signaling pathways in patient samples, protein microarrays have aided in the identification of potential protein biomarkers of disease and infection. In this perspective, the different types of full-length protein microarrays that are used in translational research are reviewed. Specific studies employing these microarrays are presented to highlight their potential in finding solutions to real clinical problems. Finally, the criteria that should be considered when developing next-generation protein microarrays are provided.
摘要
蛋白质微阵列是一种高通量技术，在转化研究中越来越多地被使用，旨在应用基础科学研究成果来增进人类健康。除了评估患者样本中的蛋白质水平、翻译后修饰和信号通路外，蛋白质微阵列还有助于识别疾病和感染的潜在蛋白质生物标志物。本文综述了转化研究中使用的不同类型的全长蛋白质微阵列。介绍了采用这些微阵列的具体研究，以突出其在解决实际临床问题方面的潜力。最后，提供了开发下一代蛋白质微阵列时应考虑的标准。
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