来自大肠杆菌的UGA抑制性tRNATrp的密码子特异性
Codon specificity of UGA suppressor tRNATrp from Escherichia coli.
作者信息
Buckingham R H, Kurland C G
出版信息
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5496-8. doi: 10.1073/pnas.74.12.5496.
Abstract
A synthetic polyribonucleotide, poly(U5,G), was used to study the codon specificity of wild-type and UGA suppressor tRNATrp from Escherichia coli. Phe (UUU) incorporation directed by this synthetic messenger is reduced somewhat by omission from the incubation mixtures of Val (GUU), Leu (UUG), or Cys (UGU). In contrast, omission of Cys stimulates Trp incorporation, and this effect is much more pronounced with the UGA suppressor tRNATrp than with wild-type tRNA. The apparent replacement of Cys by Trp is specific, because the omission of Val or Leu slightly inhibits Trp incorporation. These data suggest that the UGA suppressor tRNATrp can translate codons of the form UGN (N is any ribonucleotide). In other words, the suppressor tRNATrp translates codons that properly match two out of the three anticodon nucleotides.
摘要
一种合成的多聚核糖核苷酸聚（U5，G）被用于研究来自大肠杆菌的野生型和UGA抑制型色氨酸转运RNA（tRNATrp）的密码子特异性。由这种合成信使指导的苯丙氨酸（UUU）掺入，在孵育混合物中省略缬氨酸（GUU）、亮氨酸（UUG）或半胱氨酸（UGU）时会有所减少。相反，省略半胱氨酸会刺激色氨酸掺入，并且这种效应在UGA抑制型tRNATrp中比在野生型tRNA中更为明显。色氨酸明显取代半胱氨酸是特异性的，因为省略缬氨酸或亮氨酸会轻微抑制色氨酸掺入。这些数据表明，UGA抑制型tRNATrp可以翻译UGN形式的密码子（N是任何核糖核苷酸）。换句话说，抑制型tRNATrp翻译的密码子与三个反密码子核苷酸中的两个正确匹配。
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本文引用的文献
1
Translation of the UGA triplet in vitro by tryptophan transfer RNA's.色氨酸转运RNA在体外对UGA三联体的翻译。
J Mol Biol. 1971 Jun 14;58(2):459-68. doi: 10.1016/0022-2836(71)90363-9.
2
Tryptophan transfer RNA as the UGA suppressor.色氨酸转运RNA作为UGA抑制因子。
J Mol Biol. 1971 Jun 14;58(2):439-58. doi: 10.1016/0022-2836(71)90362-7.
3
Characterization of altered forms of glycyl transfer ribonucleic acid synthetase and the effects of such alterations on aminoacyl transfer ribonucleic acid synthesis in vivo.甘氨酰转移核糖核酸合成酶改变形式的表征及其在体内对氨酰基转移核糖核酸合成的影响。
J Bacteriol. 1970 Apr;102(1):204-12. doi: 10.1128/jb.102.1.204-212.1970.
4
Transcription and translation of prereplicative bacteriophage T4 genes in vitro.噬菌体T4复制前基因的体外转录与翻译
J Biol Chem. 1973 Aug 10;248(15):5512-9.
5
Ribosomal subunit interaction as studied by light scattering. Evidence of different classes of ribosome preparations.通过光散射研究核糖体亚基相互作用。不同类核糖体制剂的证据。
Biochemistry. 1975 Apr 22;14(8):1553-9. doi: 10.1021/bi00679a001.
6
Allosteric mechanism for codon-dependent tRNA selection on ribosomes.核糖体上密码子依赖性tRNA选择的变构机制。
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4248-51. doi: 10.1073/pnas.72.11.4248.
7
Anticodon conformation and accessibility in wild-type and suppressor tryptophan tRNA from E. coli.来自大肠杆菌的野生型和抑制型色氨酸tRNA中的反密码子构象及可及性
Nucleic Acids Res. 1976 Apr;3(4):965-75. doi: 10.1093/nar/3.4.965.
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