来自大肠杆菌的UGA抑制性tRNATrp的密码子特异性

Codon specificity of UGA suppressor tRNATrp from Escherichia coli.

作者信息

Buckingham R H, Kurland C G

出版信息

Proc Natl Acad Sci U S A. 1977 Dec;74(12):5496-8. doi: 10.1073/pnas.74.12.5496.

DOI:10.1073/pnas.74.12.5496

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431778/

Abstract

A synthetic polyribonucleotide, poly(U5,G), was used to study the codon specificity of wild-type and UGA suppressor tRNATrp from Escherichia coli. Phe (UUU) incorporation directed by this synthetic messenger is reduced somewhat by omission from the incubation mixtures of Val (GUU), Leu (UUG), or Cys (UGU). In contrast, omission of Cys stimulates Trp incorporation, and this effect is much more pronounced with the UGA suppressor tRNATrp than with wild-type tRNA. The apparent replacement of Cys by Trp is specific, because the omission of Val or Leu slightly inhibits Trp incorporation. These data suggest that the UGA suppressor tRNATrp can translate codons of the form UGN (N is any ribonucleotide). In other words, the suppressor tRNATrp translates codons that properly match two out of the three anticodon nucleotides.

摘要

一种合成的多聚核糖核苷酸聚（U5，G）被用于研究来自大肠杆菌的野生型和UGA抑制型色氨酸转运RNA（tRNATrp）的密码子特异性。由这种合成信使指导的苯丙氨酸（UUU）掺入，在孵育混合物中省略缬氨酸（GUU）、亮氨酸（UUG）或半胱氨酸（UGU）时会有所减少。相反，省略半胱氨酸会刺激色氨酸掺入，并且这种效应在UGA抑制型tRNATrp中比在野生型tRNA中更为明显。色氨酸明显取代半胱氨酸是特异性的，因为省略缬氨酸或亮氨酸会轻微抑制色氨酸掺入。这些数据表明，UGA抑制型tRNATrp可以翻译UGN形式的密码子（N是任何核糖核苷酸）。换句话说，抑制型tRNATrp翻译的密码子与三个反密码子核苷酸中的两个正确匹配。

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