Luka J, Deeb Z E, Hartmann D P, Jenson B, Pearson G R
Department of Microbiology, Georgetown University Medical Center, Washington, DC 20007.
J Natl Cancer Inst. 1988 Sep 21;80(14):1164-7. doi: 10.1093/jnci/80.14.1164.
By using monoclonal antibodies to different Epstein-Barr virus (EBV) polypeptides in combination with immunoblotting, we detected antigens associated with EBV replication in extracts from nasopharyngeal carcinoma (NPC) biopsy specimens. Major polypeptides associated with both the diffuse and the restricted components of the early antigen (EA) complex were found in extracts from nine of nine NPC biopsy specimens. Cells from an additional NPC biopsy specimen, passaged repeatedly in nude mice, were found to be positive for the major EA (restricted) polypeptide. This approach revealed that extracts from three of 14 biopsy specimens form other benign and malignant diseases also expressed these viral polypeptides. Therefore, for the first time, these results conclusively demonstrate the presence of EA polypeptides in extracts from NPC biopsy specimens. This finding provides at least a partial explanation for the reported prognostic value of antibodies to this antigen in patients with this disease.
通过使用针对不同爱泼斯坦 - 巴尔病毒（EBV）多肽的单克隆抗体并结合免疫印迹法，我们在鼻咽癌（NPC）活检标本提取物中检测到了与EBV复制相关的抗原。在9份NPC活检标本的提取物中均发现了与早期抗原（EA）复合物的弥散成分和局限成分相关的主要多肽。另外一份在裸鼠中反复传代的NPC活检标本的细胞，被发现对主要EA（局限）多肽呈阳性反应。该方法表明，来自其他良性和恶性疾病的14份活检标本中的3份提取物也表达了这些病毒多肽。因此，这些结果首次确凿地证明了NPC活检标本提取物中存在EA多肽。这一发现至少部分解释了该抗原抗体在该疾病患者中所报道的预后价值。
