亚致死高压均质处理对08中黏附机制及应激反应基因的影响
Effects of Sub-Lethal High Pressure Homogenization Treatment on the Adhesion Mechanisms and Stress Response Genes in 08.
作者信息
Braschi Giacomo, D'Alessandro Margherita, Gottardi Davide, Siroli Lorenzo, Patrignani Francesca, Lanciotti Rosalba
机构信息
Department of Agricultural and Food Sciences, University of Bologna, Cesena, Italy.
Interdepartmental Center for Industrial Agri-Food Research, University of Bologna, Cesena, Italy.
出版信息
Front Microbiol. 2021 May 28;12:651711. doi: 10.3389/fmicb.2021.651711. eCollection 2021.
Cell surface hydrophobicity (CSH) and adhesion are very important phenotypical traits for probiotics that confer them a competitive advantage for the resilience in the human gastrointestinal tract. This study was aimed to understand the effects over time of a 50 MPa hyperbaric treatment on the surface properties of 08 including CSH, autoaggregation, and adhesion (mucin layer and Caco-2 cells). Moreover, a link between the hurdle applied and the expression of genes involved in the general stress response ( and ) and adhesion processes ( and ) was evaluated. High pressure homogenization (HPH) at 50 MPa significantly increased the CSH percentage (H%), autoaggregation and adhesion on mucin of 08 cells compared with the untreated cells. Moreover, the hyperbaric hurdle induced an upregulation of the stress response genes and together with a down regulation of the and genes. Looking at the protein profile, HPH-treatment showed an increase in the number or intensity of protein bands at high and low molecular weights.
细胞表面疏水性（CSH）和黏附性是益生菌非常重要的表型特征，这些特征赋予它们在人类胃肠道中抵御外界影响的竞争优势。本研究旨在了解50 MPa高压处理对包括CSH、自聚集和黏附（黏蛋白层和Caco-2细胞）在内的8种菌株表面特性随时间的影响。此外，还评估了所施加的应激与参与一般应激反应（和）及黏附过程（和）的基因表达之间的联系。与未处理的细胞相比，50 MPa的高压均质处理（HPH）显著提高了8种菌株细胞的CSH百分比（H%）、自聚集能力及对黏蛋白的黏附性。此外，高压应激诱导了应激反应基因和的上调，同时伴随着和基因的下调。从蛋白质谱来看，HPH处理显示出高分子量和低分子量蛋白质条带数量或强度的增加。
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