FDX1 在多囊卵巢综合征（PCOS）颗粒细胞中的作用。
The role of FDX1 in granulosa cell of Polycystic ovary syndrome (PCOS).
机构信息
Department of Integrated Traditional Chinese and Western Medicine, Tongji hospital, Huazhong University of Science and Technology, Jiefang Road 1095#, 430030, Wuhan, China.
Institute of Integrated Traditional Chinese and Western Medicine, Tongji hospital, Huazhong University of Science and Technology, Jiefang Road 1095 #, 430030, Wuhan, China.
出版信息
BMC Endocr Disord. 2021 Jun 15;21(1):119. doi: 10.1186/s12902-021-00775-w.
BACKGROUND
To explore the development mechanism of PCOS and Transcriptomics was applied to seek the key gene.
METHODS
Transcriptomics marked by UID (unique identifier) technique of granulosa cell in PCOS and control women was carried out and key gene was picked up. Then the key gene in granulosa cell was measured by RT-PCR. Two PCOS models modeling with Letrozole and Testosterone Propionate were implemented and the key gene in granulosa cell of ovary was measured by immunohistochemistry to verify the relation with PCOS.
RESULTS
GO-enrich of transcriptomics concentrated in domain steroid metabolism and domain mitochondria. Different genes were sought from coexisting in both domain steroid metabolism and domain mitochondria. Finally, five different genes including CYP11A1、CYB5R1、STAR、FDX1 and AMACR were obtained. RT-PCR was implemented to furtherly verify the downregulating mRNA of FDX1 in PCOS, which showed the consistent outcome with the transcriptomics. Level of FDX1 protein in granulosa cell of antral follicle in two PCOS models was measured and decreased.
CONCLUSIONS
FDX1 was related with steroid metabolism and mitochondrial and may participate in the development of PCOS.
背景
为了探索 PCOS 的发病机制，我们应用转录组学技术寻找关键基因。
方法
采用 UID（唯一标识符）技术对 PCOS 患者和对照组妇女的颗粒细胞进行转录组学标记，筛选出关键基因，然后用 RT-PCR 检测颗粒细胞中的关键基因。建立了两种 PCOS 模型（来曲唑和丙酸睾酮），并用免疫组织化学方法检测卵巢颗粒细胞中的关键基因，以验证其与 PCOS 的关系。
结果
GO 富集的转录组学主要集中在类固醇代谢域和线粒体域。从同时存在于类固醇代谢域和线粒体域的基因中寻找差异基因。最终获得了 5 个不同的基因，包括 CYP11A1、CYB5R1、STAR、FDX1 和 AMACR。进一步用 RT-PCR 验证了 PCOS 中 FDX1mRNA 的下调，结果与转录组学一致。用免疫组织化学方法检测了两种 PCOS 模型中窦前卵泡颗粒细胞中 FDX1 蛋白的水平，发现其表达降低。
结论
FDX1 与类固醇代谢和线粒体有关，可能参与 PCOS 的发生发展。
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