Tauzin Alexandra, Nayrac Manon, Benlarbi Mehdi, Gong Shang Yu, Gasser Romain, Beaudoin-Bussières Guillaume, Brassard Nathalie, Laumaea Annemarie, Vézina Dani, Prévost Jérémie, Anand Sai Priya, Bourassa Catherine, Gendron-Lepage Gabrielle, Medjahed Halima, Goyette Guillaume, Niessl Julia, Tastet Olivier, Gokool Laurie, Morrisseau Chantal, Arlotto Pascale, Stamatatos Leonidas, McGuire Andrew T, Larochelle Catherine, Uchil Pradeep, Lu Maolin, Mothes Walther, De Serres Gaston, Moreira Sandrine, Roger Michel, Richard Jonathan, Martel-Laferrière Valérie, Duerr Ralf, Tremblay Cécile, Kaufmann Daniel E, Finzi Andrés
Centre de Recherche du CHUM, Montréal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada.
Centre de Recherche du CHUM, Montréal, QC H2X 0A9, Canada.
Cell Host Microbe. 2021 Jul 14;29(7):1137-1150.e6. doi: 10.1016/j.chom.2021.06.001. Epub 2021 Jun 4.
While the standard regimen of the BNT162b2 mRNA vaccine for SARS-CoV-2 includes two doses administered 3 weeks apart, some public health authorities are spacing these doses, raising concerns about efficacy. However, data indicate that a single dose can be up to 90% effective starting 14 days post-administration. To assess the mechanisms contributing to protection, we analyzed humoral and T cell responses three weeks after a single BNT162b2 dose. We observed weak neutralizing activity elicited in SARS-CoV-2 naive individuals but strong anti-receptor binding domain and spike antibodies with Fc-mediated effector functions and cellular CD4 T cell responses. In previously infected individuals, a single dose boosted all humoral and T cell responses, with strong correlations between T helper and antibody immunity. Our results highlight the potential role of Fc-mediated effector functions and T cell responses in vaccine efficacy. They also provide support for spacing doses to vaccinate more individuals in conditions of vaccine scarcity.
虽然用于预防新冠病毒的BNT162b2 mRNA疫苗的标准接种方案是间隔3周接种两剂，但一些公共卫生当局正在延长这些剂量之间的间隔时间，这引发了对疫苗效力的担忧。然而，数据表明，单剂疫苗在接种14天后的有效性可达90%。为了评估促成疫苗保护作用的机制，我们在接种一剂BNT162b2疫苗三周后分析了体液免疫和T细胞反应。我们观察到，在未感染过新冠病毒的个体中，疫苗引发的中和活性较弱，但产生了具有Fc介导效应功能的强效抗受体结合域和刺突抗体，以及细胞CD4 T细胞反应。在先前感染过新冠病毒的个体中，单剂疫苗增强了所有的体液免疫和T细胞反应，辅助性T细胞免疫和抗体免疫之间存在很强的相关性。我们的研究结果突出了Fc介导的效应功能和T细胞反应在疫苗效力中的潜在作用。这些结果还为在疫苗短缺的情况下延长接种剂量间隔以让更多人接种疫苗提供了支持。
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