Department of Clinical Laboratory, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250021, Shandong, China.
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, Shandong, China.
BMC Microbiol. 2021 Jun 17;21(1):183. doi: 10.1186/s12866-021-02253-8.
Lactobacilli are often recognized as beneficial partners in human microbial environments. However, lactobacilli also cause diseases in human, e.g. infective endocarditis (IE), septicaemia, rheumatic vascular disease, and dental caries. Therefore, the identification of potential pathogenic traits associated with lactobacilli will facilitate the prevention and treatment of the diseases caused by lactobacilli. Herein, we investigated the genomic traits and pathogenic potential of a novel bacterial strain Lactobacillus paracasei LP10266 which has caused a case of IE. We isolated L. paracasei LP10266 from an IE patient's blood to perform high-throughput sequencing and compared the genome of strain LP10266 with those of closely related lactobacilli to determine genes associated with its infectivity. We performed the antimicrobial susceptibility testing on strain LP10266. We assessed its virulence by mouse lethality and serum bactericidal assays as well as its serum complement- and platelet-activating ability. The biofilm formation and adherence of strain LP10266 were also studied.
Phylogenetic analysis revealed that strain LP10266 was allied with L. casei and L. paracasei. Genomic studies revealed two spaCBA pilus clusters and one novel exopolysaccharides (EPS) cluster in strain LP10266, which was sensitive to ampicillin, penicillin, levofloxacin, and imipenem, but resistant to cefuroxime, cefazolin, cefotaxime, meropenem, and vancomycin. Strain LP10266 was nonfatal and sensitive to serum, capable of activating complement 3a and terminal complement complex C5b-9 (TCC). Strain LP10266 could not induce platelet aggregation but displayed a stronger biofilm formation ability and adherence to human vascular endothelial cells (HUVECs) compared to the standard control strain L. paracasei ATCC25302.
The genome of a novel bacterial strain L. paracasei LP10266 was sequenced. Our results based on various types of assays consistently revealed that L. paracasei LP10266 was a potential pathogen to patients with a history of cardiac disease and inguinal hernia repair. Strain LP10266 showed strong biofilm formation ability and adherence, enhancing the awareness of L. paracasei infections.
乳杆菌通常被认为是人类微生物环境中的有益伙伴。然而，乳杆菌也会导致人类患病，例如感染性心内膜炎（IE）、败血症、风湿性血管疾病和龋齿。因此，鉴定与乳杆菌相关的潜在致病特征将有助于预防和治疗由乳杆菌引起的疾病。在此，我们研究了一种新型细菌菌株乳杆菌副干酪亚种 LP10266 的基因组特征和致病潜力，该菌株已导致一例 IE。我们从 IE 患者的血液中分离出 LP10266 进行高通量测序，并将菌株 LP10266 的基因组与密切相关的乳杆菌进行比较，以确定与感染性相关的基因。我们对 LP10266 进行了抗菌药物敏感性测试。我们通过小鼠致死性和血清杀菌试验以及血清补体和血小板激活能力评估其毒力。还研究了 LP10266 的生物膜形成和黏附能力。
系统发育分析显示，LP10266 菌株与干酪乳杆菌和副干酪乳杆菌关系密切。基因组研究显示 LP10266 中存在两个 spaCBA 菌毛簇和一个新型胞外多糖（EPS）簇，该菌株对氨苄西林、青霉素、左氧氟沙星和亚胺培南敏感，但对头孢呋辛、头孢唑林、头孢噻肟、美罗培南和万古霉素耐药。LP10266 菌株非致死性且对血清敏感，能够激活补体 3a 和末端补体复合物 C5b-9（TCC）。LP10266 菌株不能诱导血小板聚集，但与标准对照菌株乳杆菌副干酪亚种 ATCC25302 相比，其生物膜形成能力和对人血管内皮细胞（HUVEC）的黏附能力更强。
对新型细菌菌株乳杆菌副干酪亚种 LP10266 的基因组进行了测序。基于各种类型的检测结果一致表明，LP10266 对有心脏病和腹股沟疝修补史的患者是一种潜在的病原体。LP10266 菌株表现出较强的生物膜形成能力和黏附能力，增强了对乳杆菌感染的认识。
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