Van Dyke M W, Roeder R G, Sawadogo M
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021.
Science. 1988 Sep 9;241(4871):1335-8. doi: 10.1126/science.3413495.
Transcription of protein-encoding genes by human RNA polymerase II requires multiple ancillary proteins (transcription factors). Interactions between these proteins and the promoter DNA of a viral class II gene (the major late transcription unit of adenovirus) were investigated by enzymatic and chemical footprinting. The experiments indicated that the assembly of functionally active RNA polymerase II-containing transcription preinitiation complexes requires a complete set of transcription factors, and that both specific protein-DNA and protein-protein interactions are involved. This allows individual steps along the transcription reaction pathway to be tested directly, thus providing a basis for understanding basic transcription initiation mechanisms as well as the regulatory processes that act on them.
人类RNA聚合酶II对蛋白质编码基因的转录需要多种辅助蛋白（转录因子）。通过酶切和化学足迹法研究了这些蛋白质与病毒II类基因（腺病毒主要晚期转录单位）启动子DNA之间的相互作用。实验表明，功能活性含RNA聚合酶II转录起始前复合物的组装需要一整套转录因子，且涉及特定的蛋白质-DNA和蛋白质-蛋白质相互作用。这使得能够直接测试转录反应途径中的各个步骤，从而为理解基本转录起始机制以及作用于它们的调控过程提供了基础。
