Department of Herbology, College of Oriental Medicine, Dongshin University, 252 Daeho-dong, Naju, Jeonnam 520-714, Department of Pathology, College of Medicine, Korea University, 80 Guro-dong, Guro-gu, Seoul 152-703 and Department of Internal Medicine, College of Oriental Medicine, Dong-Eui University, Busan 614-714, Republic of Korea.
Evid Based Complement Alternat Med. 2010 Jun;7(2):239-47. doi: 10.1093/ecam/nem176. Epub 2008 Jan 7.
Reactive oxygen species (ROS) have been implicated in the pathogenesis of a wide range of acute and long-term neurodegenerative diseases. This study was undertaken to examine the efficacy of Pogostemon cablin, a well-known herb in Korean traditional medicine, on ROS-induced brain cell injury. Pogostemon cablin effectively protected human neuroglioma cell line A172 against both the necrotic and apoptotic cell death induced by hydrogen peroxide (H(2)O(2)). The effect of Pogostemon cablin was dose dependent at concentrations ranging from 0.2 to 5 mg ml(-1). Pogostemon cablin significantly prevented depletion of cellular ATP and activation of poly ADP-ribose polymerase induced by H(2)O(2). The preservation of functional integrity of mitochondria upon the treatment of Pogostemon cablin was also confirmed by 3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Furthermore, Pogostemon cablin significantly prevented H(2)O(2)-induced release of cytochrome c into cytosol. Determination of intracellular ROS showed that Pogostemon cablin might exert its role as a powerful scavenger of intracellular ROS. The present study suggests the beneficial effect of Pogostemon cablin on ROS-induced neuroglial cell injury. The action of Pogostemon cablin as a ROS-scavenger might underlie the mechanism.
活性氧（ROS）在多种急性和慢性神经退行性疾病的发病机制中都有涉及。本研究旨在研究广藿香，一种韩国传统医学中熟知的草药，对 ROS 诱导的脑细胞损伤的疗效。广藿香能有效保护人神经胶质瘤细胞系 A172 免受过氧化氢（H2O2）诱导的坏死和凋亡性细胞死亡。广藿香的作用在 0.2 至 5mg/ml 的浓度范围内呈剂量依赖性。广藿香显著防止了 H2O2 诱导的细胞内 ATP 耗竭和聚 ADP-核糖聚合酶的激活。用 3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2-H-四唑溴盐测定法还证实了处理后线粒体功能完整性的保存。此外，广藿香显著防止了 H2O2 诱导的细胞色素 c 向胞浆中的释放。细胞内 ROS 的测定表明，广藿香可能作为细胞内 ROS 的强大清除剂发挥作用。本研究表明广藿香对 ROS 诱导的神经胶质细胞损伤具有有益作用。广藿香作为 ROS 清除剂的作用可能是其作用机制。
