Liu Wenhui, Zhi Aimin
Fujian Fengjiu Biotechnology Co., Ltd. Zhangzhou China.
Food Sci Nutr. 2021 May 4;9(6):3297-3307. doi: 10.1002/fsn3.2296. eCollection 2021 Jun.
Constipation is the most common gastrointestinal complaint all over the world, and it is a risk factor of colorectal cancer. In this study, the protective of Quercetin against loperamide-induced constipation and its potential mechanism in a rat model were investigated. Results showed that Quercetin at 25 mg/kg and 50 mg/kg could significantly ( < .05) increase the intestinal transit rate, motilin, gastrin, substance P levels, and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels, and improve the gastrointestinal peristalsis of rats. In addition, the expression levels of enteric nerve-related factors, glial cell line-derived neurotrophic factor (GDNF), transient receptor potential vanilloid 1 (TRPV1), nitric oxide synthase (NOS), c-Kit, stem cell factor (SCF), and aquaporin 3 (AQP3) were examined by RT-qPCR and/or Western blot analysis. The results suggest that Quercetin relieves loperamide-induced constipation by increasing the levels of interstitial cells of Cajal markers (c-Kit and SCF), as well as AQP3. In conclusion, the present study suggested that Quercetin exerted a protective effect against loperamide-induced constipation, which may be associated with its role in regulation of multiple signal pathways.
便秘是全球最常见的胃肠道疾病，也是结直肠癌的一个危险因素。在本研究中，研究了槲皮素对洛哌丁胺诱导的便秘的保护作用及其在大鼠模型中的潜在机制。结果表明，25mg/kg和50mg/kg的槲皮素可显著（<0.05）提高肠道转运率、胃动素、胃泌素、P物质水平以及短链脂肪酸（SCFAs）浓度，降低生长抑素水平，并改善大鼠的胃肠蠕动。此外，通过RT-qPCR和/或蛋白质免疫印迹分析检测肠神经相关因子、胶质细胞源性神经营养因子（GDNF）、瞬时受体电位香草酸受体1（TRPV1）、一氧化氮合酶（NOS）、c-Kit、干细胞因子（SCF）和水通道蛋白3（AQP3）的表达水平。结果表明，槲皮素通过提高Cajal间质细胞标志物（c-Kit和SCF）以及AQP3的水平来缓解洛哌丁胺诱导的便秘。总之，本研究表明槲皮素对洛哌丁胺诱导的便秘具有保护作用，这可能与其在多条信号通路调节中的作用有关。
