Liu Yan, Yang Bo, Liu Haiying, Guo Liwei, Liu Xiaoling
School of Medicine, HeXi University, Zhangye, Gansu, 734000, China.
Department of Clinical Pharmacy, Zhangye Second People's Hospital, Zhangye, Gansu, 734000, China.
Naunyn Schmiedebergs Arch Pharmacol. 2025 Mar;398(3):2923-2931. doi: 10.1007/s00210-024-03446-4. Epub 2024 Sep 21.
Slow transit constipation (STC) seriously affects the physical and mental health of patients. While the active ingredients of traditional Chinese medicine (TCM) are widely used in the treatment of STC due to their low toxicity and side effects, the aim of this study was to investigate the effect of Lycium barbarum polysaccharide (LBP) on STC. The STC mouse model was induced by the compound diphenoxylate. Defecation, fecal moisture, and weight loss of the STC models were monitored. Gastrointestinal (GI) motility was assessed by intestinal propulsive rate, and enzyme-linked immunosorbent assay (ELISA) kits were used to analyze the levels of substance P (SP) and vasoactive intestinal peptide (VIP). The expression levels of inflammatory cytokines (Tnf-α, Il-6, and Il-1β), stem cell factor receptor (C-kit), stem cell factor (Scf), Bcl-2, Bax, and Caspase-3 were evaluated by qRT-PCR. The defecation, fecal moisture, and body weight of mice with STC were significantly improved by LBP, and LBP increased the intestinal propulsive rate of STC, increased the secretion of SP, and decreased the secretion of VIP. The intervention of LBP further suppressed the expression levels of Tnf-α, Il-6, and Il-1β in STC. LBP promoted the expression of the C-kit, Scf, and Bcl-2 and inhibited the expression of Bax and Caspase-3. LBP may alleviate symptoms of slow transit constipation (STC) and enhance gastrointestinal motility by modulating gastrointestinal hormone levels, promoting proliferation, and inhibiting the apoptosis of interstitial cells of Cajal (ICCs).
慢传输型便秘（STC）严重影响患者的身心健康。虽然中药的有效成分因其低毒性和副作用而被广泛用于STC的治疗，但本研究的目的是探讨枸杞多糖（LBP）对STC的影响。采用复方地芬诺酯诱导建立STC小鼠模型。监测STC模型的排便、粪便含水量和体重减轻情况。通过肠道推进率评估胃肠（GI）动力，并用酶联免疫吸附测定（ELISA）试剂盒分析P物质（SP）和血管活性肠肽（VIP）的水平。通过qRT-PCR评估炎性细胞因子（Tnf-α、Il-6和Il-1β）、干细胞因子受体（C-kit）、干细胞因子（Scf）、Bcl-2、Bax和Caspase-3的表达水平。LBP显著改善了STC小鼠的排便、粪便含水量和体重，提高了STC小鼠的肠道推进率，增加了SP的分泌，减少了VIP的分泌。LBP的干预进一步抑制了STC中Tnf-α、Il-6和Il-1β的表达水平。LBP促进了C-kit、Scf和Bcl-2的表达，抑制了Bax和Caspase-3的表达。LBP可能通过调节胃肠激素水平、促进增殖和抑制Cajal间质细胞（ICC）凋亡来缓解慢传输型便秘（STC）症状并增强胃肠动力。
