Vu-Duc Nam, Phung Thi Lan Anh, Le-Minh Thuy, Nguyen Lan-Anh, Nguyen-Thi Huong, Pham-Thi Loan-Ha, Doan-Thi Van-Anh, Le-Quang Huong, Nguyen-Xuan Hung, Thi Nguyen Thao, Nguyen Phuong Thanh, Chu Dinh Binh
Center for Research and Technology Transfer, Vietnam Academic of Science and Technology, 18 Hoang Quoc Viet, Hanoi 100000, Vietnam.
School of Environmental Science and Technology, Hanoi University of Science and Technology, No. 1 Dai Co Viet, Hanoi 100000, Vietnam.
J Anal Methods Chem. 2021 May 26;2021:6641326. doi: 10.1155/2021/6641326. eCollection 2021.
Polycyclic aromatic hydrocarbons (PAHs), the family of organic contaminations, have been shown to have negative effects on human health. However, until now, the comprehension on occurrence, distribution, and risk assessment of human exposure to PAHs has been limited in Vietnam. In this work, a capillary gas chromatography coupled with electron impact ionization tandem mass spectrometry (GC-EI-MS/MS) has been introduced for analysis of 16 PAHs in some particulate matter samples. PAHs have been separated on the TG 5 ms capillary gas chromatographic column and detected by tandem mass spectrometry in multiple reaction monitoring mode. The PAHs in the particulate matter (PM 2.5 and PM 10) samples were extracted by ultrasonic-assisted liquid extraction and cleaned up by an acidic silica gel solid phase extraction. The linearity range of all analyzed PAHs was from 5 to 2000 ng mL with ≥0.9990. Limit of detection (LOD) of PAHs in particulate matter sample was from 0.001 ng m (Br-Naph) to 0.276 ng m (Fln). The recovery of PAHs was investigated by international proficiency testing samples. The recoveries of PAHs in proficiency testing sample ranged from 79.3% (Chr) to 109.8% (IcdP). The in-house validated GC-EI-MS/MS method was then applied to analysis of some particulate matter samples that were collected in the Hanoi areas. The total concentrations of PAHs in several brands of samples collected from Hanoi were found in the range of 226.3 ng m-706.43 ng m. Among the studied compounds, naphthalene was found at high frequency and ranged from 106.5 ng m to 631.1 ng m. The main distribution of the PAHs in particulate matter samples was two-ring and three-ring compounds.
多环芳烃（PAHs）作为一类有机污染物，已被证明会对人体健康产生负面影响。然而，截至目前，越南对人类接触多环芳烃的发生、分布及风险评估的了解仍较为有限。在本研究中，引入了毛细管气相色谱-电子轰击电离串联质谱联用技术（GC-EI-MS/MS）来分析一些颗粒物样品中的16种多环芳烃。多环芳烃在TG 5 ms毛细管气相色谱柱上实现分离，并采用串联质谱的多反应监测模式进行检测。颗粒物（PM 2.5和PM 10）样品中的多环芳烃通过超声辅助液液萃取进行提取，并用酸性硅胶固相萃取进行净化。所有分析的多环芳烃的线性范围为5至2000 ng/mL，相关系数≥0.9990。颗粒物样品中多环芳烃的检出限为0.001 ng/m³（溴萘）至0.276 ng/m³（荧蒽）。通过国际能力验证样品对多环芳烃的回收率进行了研究。能力验证样品中多环芳烃的回收率范围为79.3%（苯并[b]荧蒽）至109.8%（异佛尔酮）。随后，将内部验证的GC-EI-MS/MS方法应用于分析在河内地区采集的一些颗粒物样品。从河内采集的几个品牌样品中多环芳烃的总浓度范围为226.3 ng/m³至706.43 ng/m³。在所研究的化合物中，萘的检出频率较高，范围为106.5 ng/m³至631.1 ng/m³。颗粒物样品中多环芳烃的主要分布为二环和三环化合物。
