Cheng Zhangrui, Buggiotti Laura, Salavati Mazdak, Marchitelli Cinzia, Palma-Vera Sergio, Wylie Alistair, Takeda Haruko, Tang Lijing, Crowe Mark A, Wathes D Claire
Royal Veterinary College, Hatfield, Herts, UK.
The Roslin Institute, Royal (Dick) School of Veterinary Studies, Easter Bush Campus, Midlothian, UK.
Mol Biol Rep. 2021 May;48(5):4611-4623. doi: 10.1007/s11033-021-06494-8. Epub 2021 Jun 19.
Bovine mastitis, an inflammatory disease of the mammary gland, is classified as subclinical or clinical. Circulating neutrophils are recruited to the udder to combat infection. We compared the transcriptomic profiles in circulating leukocytes between healthy cows and those with naturally occurring subclinical or clinical mastitis. Holstein Friesian dairy cows from six farms in EU countries were recruited. Based on milk somatic cell count and clinical records, cows were classified as healthy (n = 147), subclinically (n = 45) or clinically mastitic (n = 22). Circulating leukocyte RNA was sequenced with Illumina NextSeq single end reads (30 M). Differentially expressed genes (DEGs) between the groups were identified using CLC Genomics Workbench V21, followed by GO enrichment analysis. Both subclinical and clinical mastitis caused significant changes in the leukocyte transcriptome, with more intensive changes attributed to clinical mastitis. We detected 769 DEGs between clinical and healthy groups, 258 DEGs between subclinical and healthy groups and 193 DEGs between clinical and subclinical groups. Most DEGs were associated with cell killing and immune processes. Many upregulated DEGs in clinical mastitis encoded antimicrobial peptides (AZU1, BCL3, CAMP, CATHL1, CATHL2, CATHL4,CATHL5, CATHL6, CCL1, CXCL2, CXCL13, DEFB1, DEFB10, DEFB4A, DEFB7, LCN2, PGLYRP1, PRTN3, PTX3, S100A8, S100A9, S100A12, SLC11A1, TF and LTF) which were not upregulated in subclinical mastitis. The use of transcriptomic profiles has identified a much greater up-regulation of genes encoding antimicrobial peptides in circulating leukocytes of cows with naturally occurring clinical compared with subclinical mastitis. These could play a key role in combatting disease organisms.
牛乳腺炎是一种乳腺的炎症性疾病，分为亚临床型或临床型。循环中的中性粒细胞被募集到乳房以对抗感染。我们比较了健康奶牛与自然发生亚临床型或临床型乳腺炎奶牛循环白细胞中的转录组谱。招募了来自欧盟国家六个农场的荷斯坦弗里生奶牛。根据牛奶体细胞计数和临床记录，奶牛被分为健康组（n = 147）、亚临床型组（n = 45）或临床型乳腺炎组（n = 22）。使用Illumina NextSeq单端读数（30M）对循环白细胞RNA进行测序。使用CLC Genomics Workbench V21识别组间差异表达基因（DEG），随后进行基因本体（GO）富集分析。亚临床型和临床型乳腺炎均导致白细胞转录组发生显著变化，临床型乳腺炎引起的变化更为强烈。我们在临床型与健康组之间检测到769个DEG，亚临床型与健康组之间检测到258个DEG，临床型与亚临床型组之间检测到193个DEG。大多数DEG与细胞杀伤和免疫过程相关。临床型乳腺炎中许多上调的DEG编码抗菌肽（AZU1、BCL3、CAMP、CATHL1、CATHL2、CATHL4、CATHL5、CATHL6、CCL1、CXCL2、CXCL13、DEFB1、DEFB10、DEFB4A、DEFB7、LCN2、PGLYRP1、PRTN3、PTX3、S100A8、S100A9、S100A12、SLC11A1、TF和LTF），而这些在亚临床型乳腺炎中并未上调。转录组谱的使用已确定，与亚临床型乳腺炎相比，自然发生临床型乳腺炎的奶牛循环白细胞中编码抗菌肽的基因上调幅度要大得多。这些抗菌肽可能在对抗致病生物中起关键作用。
