虹鳟（鲑鱼）肾小球对血清蛋白的选择通透性

Glomerular permselectivity to serum proteins in rainbow trout (Salmo gairdneri).

作者信息

Elger B, Rühs H, Hentschel H

机构信息

Zentrum Innere Medizin, Medizinische Hochschule Hannover, Federal Republic of Germany.

出版信息

Am J Physiol. 1988 Sep;255(3 Pt 2):R418-23. doi: 10.1152/ajpregu.1988.255.3.R418.

DOI:10.1152/ajpregu.1988.255.3.R418

PMID:3414836

Abstract

Changes in glomerular filtration rate (GFR) were induced in the unanesthetized rainbow trout by short-term adaptation from freshwater (FW) to brackish water (BW, 50% seawater) to investigate the effect of altered glomerular function on the glomerular permselectivity to endogenous serum proteins in vivo. Protein patterns of serum and urine were obtained by nondenaturing polyacrylamide gradient gel electrophoresis. In the serum, high-molecular-weight proteins dominate by 83.1 +/- 8.16%, and total serum protein concentrations were 3.25 +/- 0.32 (FW) and 3.12 +/- 0.16 g/100 ml (BW). The urinary protein concentration increased from 16.0 +/- 1.41 (FW) to 42.8 +/- 11.0 mg/100 ml (BW), whereas GFR and urine flow rate, both linearly related, decreased from 4.55 +/- 0.34 (FW) to 1.84 +/- 0.11 (BW) ml.h-1.kg-1 and from 2.31 +/- 0.19 (FW) to 0.56 +/- 0.02 (BW) ml.h-1.kg-1, respectively. The fractional clearance of protein increased exponentially at decreased GFR. This suggests increased glomerular filtration of serum proteins due to altered glomerular hemodynamics.

摘要

通过将未麻醉的虹鳟鱼从淡水（FW）短期适应到半咸水（BW，50%海水）来诱导肾小球滤过率（GFR）的变化，以研究肾小球功能改变对体内肾小球对内源性血清蛋白的选择通透性的影响。通过非变性聚丙烯酰胺梯度凝胶电泳获得血清和尿液的蛋白质图谱。在血清中，高分子量蛋白质占主导，比例为83.1±8.16%，血清总蛋白浓度在淡水组为3.25±0.32 g/100 ml，在半咸水组为3.12±0.16 g/100 ml。尿蛋白浓度从淡水组的16.0±1.41 mg/100 ml增加到半咸水组的42.8±11.0 mg/100 ml，而与两者均呈线性相关的GFR和尿流率分别从淡水组的4.55±0.34 ml·h⁻¹·kg⁻¹降至半咸水组的1.84±0.11 ml·h⁻¹·kg⁻¹，以及从淡水组的2.31±0.19 ml·h⁻¹·kg⁻¹降至半咸水组的0.56±0.02 ml·h⁻¹·kg⁻¹。随着GFR降低，蛋白质的分数清除率呈指数增加。这表明由于肾小球血流动力学改变，血清蛋白的肾小球滤过增加。