虹鳟鱼（Salmo gairdneri）中血管紧张素与单肾单位肾小球功能

Angiotensin and single nephron glomerular function in the trout Salmo gairdneri.

作者信息

Brown J A, Oliver J A, Henderson I W, Jackson B A

出版信息

Am J Physiol. 1980 Nov;239(5):R509-14. doi: 10.1152/ajpregu.1980.239.5.R509.

DOI:10.1152/ajpregu.1980.239.5.R509

PMID:7001929

Abstract

Glomerular filtration rate (GFR), urine flow (UV), renal tubular transport maximum for glucose (TMG), and single nephron glomerular filtration rate (SNGFR), determined in anesthetized norepinephrine-infused trout (Salmo gairdneri), were 18.51 +/- 5.78 microliter x min-1 x kg body wt-1, 5.31 +/- 1.38 microliter x min-1 x kg body wt-1, 105.21 +/- 46.84 microgram x min-1 x kg body wt-1, and 3.74 +/- 1.12 nl/min, respectively, when in seawater (SW) and 140.39 +/- 17.24, 76.38 +/- 10.41, 626.16 +/- 77.46, and 1.31 +/- 0.20 in freshwater (FW). Angiotensin II infusions, to reduce UV, GFR, and TMG by 50%, had no effect on the average SNGFR of FW trout, but reduced that of SW fish to 1.42 +/- 0.19 nl/min. Infusions of 20% ferrocyanide, visualized as Prussian blue (PB), identified three glomerular populations: filtering (F) with PB in glomerular vessels and tubular lumen; nonfiltering (NF)--PB in glomerular vessels only; nonperfused (NP)--no PB associated with the nephron. SW and FW kidneys contained about 40% NF tubules. In FW, 45% were F tubules compared with 5% in SW, whereas NP tubules comprised 51% of SW tubules and 13% of FW. During angiotensin II infusions the distributions were 9% and 46% NF in FW and 6% F and 12% NF in SW trout.

摘要

在经麻醉并注入去甲肾上腺素的虹鳟鱼（Salmo gairdneri）中测定的肾小球滤过率（GFR）、尿流率（UV）、肾小管对葡萄糖的转运最大值（TMG）和单肾单位肾小球滤过率（SNGFR），在海水中（SW）时分别为18.51±5.78微升·分钟⁻¹·千克体重⁻¹、5.31±1.38微升·分钟⁻¹·千克体重⁻¹、105.21±46.84微克·分钟⁻¹·千克体重⁻¹和3.74±1.12纳升/分钟，而在淡水中（FW）时分别为140.39±17.24、76.38±10.41、626.16±77.46和1.31±0.20。输注血管紧张素II使UV、GFR和TMG降低50%，对淡水虹鳟鱼的平均SNGFR没有影响，但使海水虹鳟鱼的SNGFR降至1.42±0.19纳升/分钟。输注20%的亚铁氰化物，以普鲁士蓝（PB）显示，确定了三种肾小球类型：滤过型（F），肾小球血管和肾小管腔中有PB；非滤过型（NF）——仅肾小球血管中有PB；未灌注型（NP）——肾单位无PB相关。海水和淡水肾脏中约40%为NF肾小管。在淡水中，45%为F肾小管，而海水中为5%，而NP肾小管在海水中占51%，在淡水中占13%。在输注血管紧张素II期间，淡水虹鳟鱼中NF的分布为9%和46%，海水虹鳟鱼中F为6%，NF为12%。