Brown J A, Taylor S M, Gray C J
Cell Tissue Res. 1983;230(1):205-18. doi: 10.1007/BF00216040.
The epithelial ultrastructure of the glomerulus of fresh- and sea-water adapted rainbow trout, Salmo gairdneri was examined by scanning and transmission electron microscopy after in vivo-perfusion fixation. Adaptation to seawater for a period of up to 9 weeks was not associated with a change in glomerular size, although after one year in seawater both glomeruli and their capsules were significantly smaller than the glomeruli of freshwater adapted fish. In freshwater adapted trout the epithelial ultrastructure conformed to the general vertebrate pattern, with rounded podocytes, well-defined primary processes and interdigitating pedicels. Adaptation to seawater was associated with a marked increase in the frequency of cytoplasmic microprojections. In many glomeruli there was a flattening of the podocytes, broadening of the primary processes, and areas of closely-packed pedicels were common. These observations are discussed in the light of previous studies on the mammalian kidney and the changes of single nephron function known to be associated with adaptation of trout to increased environmental salinities.
在体内灌注固定后，通过扫描电子显微镜和透射电子显微镜对适应淡水和海水的虹鳟（Salmo gairdneri）肾小球的上皮超微结构进行了检查。长达9周的海水适应期与肾小球大小的变化无关，尽管在海水中生活一年后，肾小球及其包膜均明显小于适应淡水的鱼的肾小球。在适应淡水的鳟鱼中，上皮超微结构符合一般脊椎动物的模式，足细胞呈圆形，初级突起清晰，足突相互交错。适应海水与细胞质微突起频率显著增加有关。在许多肾小球中，足细胞变平，初级突起变宽，紧密排列的足突区域很常见。根据先前对哺乳动物肾脏的研究以及已知与鳟鱼适应环境盐度增加相关的单个肾单位功能变化，对这些观察结果进行了讨论。
