Comprehensive analysis of differentially expressed long noncoding RNAs, miRNAs and mRNAs in breast cancer brain metastasis.

To delineate the transcriptomic landscape and potential molecular mechanisms of breast cancer brain metastasis (BCBM). Whole-transcriptome sequencing was performed to identify long noncoding RNA (lncRNA), miRNA and mRNA expression profiles associated with BCBM. A total of 739 differentially expressed lncRNAs, 115 differentially expressed miRNAs and 5749 differentially expressed mRNAs were identified in 231-BR cells compared with MDA-MB-231 cells. Real-time quantitative PCR results revealed the expression levels of candidate molecules were consistent with their correspondence RNA-seq data. Protein-protein interaction analysis identified some hub genes associated with BCBM, such as , and . LncRNA-miRNA-mRNA network highlighted a potential mechanism of BCBM in which lncRNA and RP11-169F17.1 sponging hsa-miR-501-5p to regulate the expression of , and . This study provides a framework for better understanding molecular mechanisms of BCBM.