Moshaverinia Maryam, Sahmeddini Sarina, Lavee Fatemeh, Zareshahrabadi Zahra, Zomorodian Kamiar
Department of Oral and Maxillofacial Disease, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Biomed Res Int. 2022 Sep 12;2022:9744153. doi: 10.1155/2022/9744153. eCollection 2022.
Oral infections associated with a wide diversity of microorganisms, including bacteria and yeasts, occur frequently in humans, affecting the whole oral cavity and well-being. Oral pathogens easily grow and propagate in the oral cavity, leading to the formation of dental plaque on both soft and hard tissue. The oral cavity contains up to 700 different species of microorganisms, which and are the most common organisms. Oral diseases continue to increase despite the best efforts of the medical and scientific communities. During the past decades, drug resistance to common antibiotics used in the treatment of oral infections has been raised to high levels worldwide. To overcome such resistance, there is a growing tendency to use herbal medicine as alternative. This study was conducted to find out the chemical constitution of () essential oil and to determine its antimicrobial and anti-biofilm efficacy against common oral pathogens.
The chemical compositions of the essential oil distilled from plants were analyzed using gas chromatography/mass spectrometry (GC/MS). Antimicrobial susceptibility testing against common , , , and strains was assessed by broth microdilution in 96-well plates as suggested by the Clinical and Laboratory Standards Institute (CLSI) methods. Biofilm growth and development were assessed using XTT reduction assay.
Based on the GC/MS test results, (67.75%) followed by (E-) (7.27%) was the main component of this essential oil inhibited the growth of examined microbial pathogens at concentrations of 0.031-16 L/mL. Also, the essential oil showed biofilm inhibition of greater than 95% in the concentration of 8 L/mL against all tested bacterial strains as well as ( value < 0.05).
Considering the significant antimicrobial activities of , this essential oil has the potential to be used as further antimicrobial and anti-biofilm pharmaceutical products in the control and treatment of oral infections.
与包括细菌和酵母菌在内的多种微生物相关的口腔感染在人类中频繁发生，影响整个口腔及健康状况。口腔病原体易于在口腔中生长和繁殖，导致软硬组织上形成牙菌斑。口腔中含有多达700种不同的微生物物种，其中 和 是最常见的微生物。尽管医学和科学界已做出最大努力，但口腔疾病仍在不断增加。在过去几十年中，全球范围内用于治疗口腔感染的常见抗生素的耐药性已升至很高水平。为克服这种耐药性，使用草药作为替代品的趋势日益增加。本研究旨在查明 （）精油的化学成分，并确定其对常见口腔病原体的抗菌和抗生物膜功效。
采用气相色谱/质谱联用仪（GC/MS）分析从 植物中蒸馏出的精油的化学成分。按照临床和实验室标准协会（CLSI）的方法建议，通过在96孔板中进行肉汤微量稀释法评估对常见的 、 、 和 菌株的抗菌药敏试验。使用XTT还原试验评估生物膜的生长和发育情况。
根据GC/MS测试结果， （67.75%）其次是 （E-）（7.27%）是该精油的主要成分，在浓度为0.031 - 16 L/mL时抑制所检测微生物病原体的生长。此外，该精油在浓度为8 L/mL时对所有测试细菌菌株以及 均显示出大于95%的生物膜抑制作用（ 值<0.05）。
考虑到 的显著抗菌活性，这种精油有潜力作为进一步的抗菌和抗生物膜药物产品用于口腔感染的控制和治疗。
