开发一种用于从鸡肉中分离的新型专业培养方法。
Development of a novel and specialized cultivation method for isolating from chicken meat.
作者信息
Akhlaghi H, Emadi Chashmi S H, Jebelli Javan A
机构信息
DVM Student, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Department of Clinical Science, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
出版信息
Iran J Vet Res. 2021 Winter;22(1):76-80. doi: 10.22099/ijvr.2020.38193.5560.
BACKGROUND
It has become established that could be isolated from raw chicken meat.
AIMS
This study was aimed to develop a novel culture method (protocol B) to isolate from chicken meat by adding some modifications to the traditional culture method (protocol A), and as a consequence to compare their sensitivity, specificity, and the accuracy of these methods with polymerase chain reaction (PCR) test.
METHODS
400 chicken meat samples were collected from various retail markets and supermarkets. Each sample was processed by protocol A, protocol B, and PCR test.
RESULTS
Out of 400 samples, 77 (19.25%), and 163 (40.75%) were culture-positive by protocol A and protocol B, respectively. Using PCR test as a gold standard, 196 (49%) samples were identified as . The specificity for both protocols was determined 100%, while the sensitivity of protocol B and protocol A was assessed 83% and 39%, respectively. Also, the higher and lower accuracy belonged to protocol B (92%) and protocol A (70%), respectively.
CONCLUSION
The methodology designed herein can provide a suitable, approximately sensitive, specific, and accurate method to cultivate from chicken meat.
背景
现已证实可从生鸡肉中分离出[具体物质未给出]。
目的
本研究旨在开发一种新的培养方法（方案B），通过对传统培养方法（方案A）进行一些改进，从鸡肉中分离出[具体物质未给出]，并将这些方法的敏感性、特异性及准确性与聚合酶链反应（PCR）检测进行比较。
方法
从各个零售市场和超市收集400份鸡肉样本。每个样本分别采用方案A、方案B及PCR检测进行处理。
结果
在400份样本中，分别有77份（19.25%）和163份（40.75%）通过方案A和方案B培养呈阳性。以PCR检测作为金标准，196份（49%）样本被鉴定为[具体物质未给出]。两种方案的特异性均为100%，而方案B和方案A的敏感性分别为83%和39%。此外，方案B（92%）和方案A（70%）的准确性分别为较高和较低。
结论
本文设计的方法能够提供一种合适的、近似灵敏、特异且准确的从鸡肉中培养[具体物质未给出]的方法。
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