Abbasi Fariba, Samaei Mohammad Reza, Hashemi Hassan, Savardashtaki Amir, Azhdarpoor Abooalfazl, Fallahi Mohammad Javad, Jalili Mahrokh, Billet Sylvain
Department of Environmental Health Engineering, Shiraz University of medical science, Shiraz, Iran.
Department of Medical Biotechnology, Shiraz University of medical science, Shiraz, Iran.
J Environ Health Sci Eng. 2021 Apr 30;19(1):985-995. doi: 10.1007/s40201-021-00663-4. eCollection 2021 Jun.
Less attention had been paid to cell toxicity of the various synthesis methods of nanoparticles, this study investigated the effect of the calcination temperature(CT) on the crystallization of SiO nanoparticles(NPs), cell proliferation(CP), and cellular uptake(CU) in MRC-5. In this study, parameters were adjusted as CT(70-1000 °C), calcination time(2, 12, and 24 h), and catalyst feed rate(0.01, 0.05, and 0.1 mL.min). CP was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) test after a 24-h exposure. The CU was achieved using ICP-MS. Results were analyzed using MATLAB2018. Results revealed that the size of synthesized particles was lower than 50 nm and, the XRD peak varied from 21 to 30° during the increase in CT. FTIR spectra confirmed the existence of Si-O and Si-Cl bonds. The maximum level of crystallization was at 1000 °C. CP decreased with the rise in the concentration of NPs( < 0.05), as well as an increase in feed rate. A positive relationship between increased crystallization and decreased CP(R = 0.78) was seen, while such a trend was not observed in calcination time. The suggested structure in this study was 4:10:1 with R = 0.97, R = 0.97, RMSE = 0.25, and MSE = 0.003. Furthermore, the CU rate increased with the rise in CT and calcination time. The maximum and minimum CU levels were related to NPs calcinated in 1000 °C-24 h and 350 °C-2 h, respectively. As a consequence, the most toxicity of SiO NPs was related to the crystalline NP. Therefore, the increase in CT and the calcination time were significant factors affecting on crystallization of SiO NPs, CP of lung cell, as well as CU of SiO.
The online version contains supplementary material available at 10.1007/s40201-021-00663-4.
人们对纳米颗粒各种合成方法的细胞毒性关注较少，本研究调查了煅烧温度（CT）对MRC-5细胞中SiO纳米颗粒（NPs）结晶、细胞增殖（CP）和细胞摄取（CU）的影响。在本研究中，调整的参数为CT（70 - 1000°C）、煅烧时间（2、12和24小时）以及催化剂进料速率（0.01、0.05和0.1 mL·min）。暴露24小时后，通过3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐（MTT）试验测定CP。使用电感耦合等离子体质谱法（ICP - MS）实现CU。使用MATLAB2018分析结果。结果显示，合成颗粒的尺寸小于50 nm，并且在CT升高期间XRD峰在21°至30°之间变化。傅里叶变换红外光谱（FTIR）证实了Si - O和Si - Cl键的存在。最大结晶水平出现在1000°C。CP随着NPs浓度的升高（<0.05）以及进料速率的增加而降低。观察到结晶增加与CP降低之间存在正相关（R = 0.78），而在煅烧时间方面未观察到这种趋势。本研究中建议的结构为4:10:1，R = 0.97，R = 0.97，均方根误差（RMSE）= 0.25，均方误差（MSE）= 0.003。此外，CU率随着CT和煅烧时间的增加而增加。最大和最小CU水平分别与在1000°C - 24小时和350°C - 2小时煅烧的NPs相关。因此，SiO NPs的最大毒性与结晶态NP有关。所以，CT的增加和煅烧时间是影响SiO NPs结晶、肺细胞CP以及SiO CU的重要因素。
在线版本包含可在10.1007/s40201 - 021 - 00663 - 4获取的补充材料。
