Nakazawa T, Sato K, Tsuchiya J
College of Medical Care and Technology, Gunma University, Japan.
Ann Allergy. 1988 Sep;61(3):214-5.
Release fluoroimmunoassay (RFIA), a new method of enzyme immunoassay (ELISA), was employed for the detection of specific IgE antibody in patients allergic to Japanese cedar pollen. RFIA clearly discriminated between allergic patients and healthy subjects. Further, RFIA was able to detect the minute IgE antibody at the dilution range of 1/32 of patient's serum. Correlation between the relative fluorescent unit values and the RAST values of sera from 17 patients and seven healthy subjects was very high. The correlation coefficient was 0.87. These findings show that the RFIA has an advantage over the other procedures for the diagnosis of immediate-type hypersensitivity as well as the diagnosis of Japanese cedar pollen.
释放荧光免疫测定法（RFIA）是酶免疫测定法（ELISA）的一种新方法，用于检测对日本雪松花粉过敏患者的特异性IgE抗体。RFIA能明显区分过敏患者和健康受试者。此外，RFIA能够在患者血清1/32的稀释范围内检测到微量IgE抗体。17例患者和7例健康受试者血清的相对荧光单位值与放射性变应原吸附试验（RAST）值之间的相关性非常高。相关系数为0.87。这些结果表明，RFIA在速发型超敏反应诊断以及日本雪松花粉诊断方面比其他方法具有优势。
