Institute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
Sabinsa Corporation, East Windsor, New Jersey 08520, United States.
J Agric Food Chem. 2021 Jun 30;69(25):7093-7103. doi: 10.1021/acs.jafc.1c02783. Epub 2021 Jun 21.
The worldwide prevalence of obesity has significantly increased over the past few decades. It is currently believed that obesity is a risk factor for developing inflammatory bowel disease. Pterostilbene (PTS), a naturally occurring stilbene from blueberries, is known to have anticancer, anti-inflammation, antifibrosis, and antiobesity effects. The preventive effect of PTS on the susceptibility of high-fat diet (HFD) to dextran sulfate sodium (DSS)-induced colitis in mice was investigated. Beginning at 5 weeks of age, C57BL/6J mice were fed a normal diet, 50% HFD alone, or containing PTS, and DSS (2.5%, w/v) was given in drinking water at week 9 and week 11. The results demonstrated that PTS significantly attenuated HFD and DSS-induced plasma interleukin-6 accumulation. Moreover, PTS suppressed HFD/DSS-induced formation of aberrant crypt foci and reduced the colon weight-to-length ratio in HFD/DSS-induced colitis mice. Furthermore, PTS inhibited interleukin-1β (IL-1β), the C/EBP homologous protein (CHOP), cyclooxygenase-2, and transforming growth factor beta-1 (TGF-β1)/mothers against decapentaplegic homolog 2 expression and maintained mucin2 (Muc2) and E-cadherin expressions. In addition, post-treatment with PTS also decreased the colon weight-to-length ratio and loss of Muc2. Moreover, the CHOP, IL-1β, matrix metalloproteinase-2, and TGF-β1 expressions were significantly decreased in HFD/DSS-induced colitis mice after post-treatment with PTS. In conclusion, the results of the present study suggest that PTS is of significant interest for the prevention of HFD/DSS-induced colitis in C57BL/6J mice.
在过去的几十年中，全球肥胖的患病率显著增加。目前认为肥胖是引发炎症性肠病的一个风险因素。紫檀芪（PTS）是一种存在于蓝莓中的天然二苯乙烯，具有抗癌、抗炎、抗纤维化和抗肥胖的作用。本研究旨在探讨紫檀芪对高脂肪饮食（HFD）诱导的葡聚糖硫酸钠（DSS）结肠炎易感性的预防作用。从 5 周龄开始，C57BL/6J 小鼠分别喂食正常饮食、50%HFD 单独喂养或添加 PTS 的 HFD，并在第 9 周和第 11 周在饮用水中给予 DSS（2.5%，w/v）。结果表明，紫檀芪显著减轻了 HFD 和 DSS 诱导的血浆白细胞介素-6 积累。此外，紫檀芪抑制了 HFD/DSS 诱导的异常隐窝形成，并降低了 HFD/DSS 诱导的结肠炎小鼠的结肠重量与长度比。此外，紫檀芪抑制了白细胞介素-1β（IL-1β）、C/EBP 同源蛋白（CHOP）、环氧化酶-2 和转化生长因子-β1（TGF-β1）/母亲对抗 decapentaplegic 同源物 2 的表达，并维持粘蛋白 2（Muc2）和 E-钙黏蛋白的表达。此外，紫檀芪的后期治疗也降低了结肠重量与长度比和 Muc2 的丧失。此外，紫檀芪的后期治疗还显著降低了 HFD/DSS 诱导的结肠炎小鼠的 CHOP、IL-1β、基质金属蛋白酶-2 和 TGF-β1 的表达。综上所述，本研究结果表明，紫檀芪对 C57BL/6J 小鼠的 HFD/DSS 诱导的结肠炎具有重要的预防作用。
