Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, India.
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, India.
Arch Microbiol. 2021 Sep;203(7):4539-4548. doi: 10.1007/s00203-021-02426-z. Epub 2021 Jun 21.
Difficulties in controlling the soil-borne plant pathogenic fungus Sclerotium rolfsii favoured the analysis of its suppressive soil for better understanding. In the present study, culture-independent molecular technique was used to analyse the bacterial communities of suppressive soil and conducive soil. Hence, metagenomic DNAs from both kinds of soils were directly extracted and their sequence polymorphism was analysed by targeting hypervariable domains, V4 + V5, of the 16S rRNA gene. The results of 16S rRNA gene-driven bacterial community diversity analysis along with soil physicochemical and biological properties clearly discriminated S. rolfsii suppressive soil from conducive soil. The dominant phylogenetic group of suppressive soil is Actinobacteria followed by Proteobacteria. The other groups include Acidobacteria, Firmicutes and Cyanobacteria. In contrast, conducive soil had very few Actinobacterial sequences and was dominated by Gamma- and Betaproteobacteria. Based on the relative proportion of different bacterial communities, their diversity and species richness were observed more in suppressive soil than in conducive soil. The present study identifies the dominant bacterial community which shares S. rolfsii suppressiveness.
控制土传植物病原真菌立枯丝核菌的困难促使人们对其抑制土壤进行分析，以更好地了解它。在本研究中，采用非培养的分子技术分析了抑制土壤和促进土壤中的细菌群落。因此，直接从这两种土壤中提取了宏基因组 DNA，并通过靶向 16S rRNA 基因的高变区 V4+V5 来分析其序列多态性。16S rRNA 基因驱动的细菌群落多样性分析结果以及土壤理化和生物学特性清楚地区分了立枯丝核菌抑制土壤和促进土壤。抑制土壤的主要优势菌门是放线菌，其次是变形菌门。其他群包括酸杆菌门、厚壁菌门和蓝细菌门。相比之下，促进土壤中的放线菌序列很少，主要由γ-和β-变形菌门组成。基于不同细菌群落的相对比例，抑制土壤中的多样性和丰富度均高于促进土壤。本研究确定了与立枯丝核菌抑制性相关的主要细菌群落。
