Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.
Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Madrid, Spain.
Sci Rep. 2021 Jun 21;11(1):12996. doi: 10.1038/s41598-021-92468-6.
The success in the response of a plant to a pest depends on the regulatory networks that connect plant perception and plant response. Meta-analyses of transcriptomic responses are valuable tools to discover novel mechanisms in the plant/herbivore interplay. Considering the quantity and quality of available transcriptomic analyses, Arabidopsis thaliana was selected to test the ability of comprehensive meta-analyses to disentangle plant responses. The analysis of the transcriptomic data showed a general induction of biological processes commonly associated with the response to herbivory, like jasmonate signaling or glucosinolate biosynthesis. However, an uneven induction of many genes belonging to these biological categories was found, which was likely associated with the particularities of each specific Arabidopsis-herbivore interaction. A thorough analysis of the responses to the lepidopteran Pieris rapae and the spider mite Tetranychus urticae highlighted specificities in the perception and signaling pathways associated with the expression of receptors and transcription factors. This information was translated to a variable alteration of secondary metabolic pathways. In conclusion, transcriptomic meta-analysis has been revealed as a potent way to sort out relevant physiological processes in the plant response to herbivores. Translation of these transcriptomic-based analyses to crop species will permit a more appropriate design of biotechnological programs.
植物对害虫的反应成功与否取决于连接植物感知和植物反应的调控网络。对转录组反应的元分析是发现植物/草食动物相互作用中新型机制的有价值的工具。考虑到可用转录组分析的数量和质量，选择拟南芥来测试综合元分析分离植物反应的能力。转录组数据分析显示，与对草食性的反应通常相关的生物过程普遍诱导，如茉莉酸信号或硫代葡萄糖苷生物合成。然而，属于这些生物类别的许多基因的不均匀诱导被发现，这可能与每种特定的拟南芥-草食动物相互作用的特殊性有关。对鳞翅目菜粉蝶和叶螨的反应的彻底分析突出了与受体和转录因子表达相关的感知和信号通路的特异性。这些信息转化为次生代谢途径的可变性改变。总之，转录组元分析已被证明是一种有效的方法，可以梳理植物对草食动物反应中的相关生理过程。将这些基于转录组的分析转化为作物物种，将允许更适当地设计生物技术计划。
