Department of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy Street, Newark, DE 19716 United States.
Department of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy Street, Newark, DE 19716 United States.
Curr Opin Biotechnol. 2021 Oct;71:41-48. doi: 10.1016/j.copbio.2021.06.002. Epub 2021 Jun 19.
The importance of bioimaging and biosensing has been clear with the onset of the COVID-19 pandemic. In addition to viral detection, detection of tumors, glucose levels, and microbes is necessary for improved disease treatment and prevention. Bionanoparticles, such as extracellular vesicles and protein nanoparticles, are ideal platforms for biosensing and bioimaging applications because of their propensity for high density surface functionalization and large loading capacity. Scaffolding large numbers of sensing modules and detection modules onto bionanoparticles allows for enhanced analyte affinity and specificity as well as signal amplification for highly sensitive detection even at low analyte concentrations. Here we demonstrate the potential of bionanoparticles for bioimaging and biosensing by highlighting recent examples in literature that utilize protein nanoparticles and extracellular vesicles to generate highly sensitive detection devices with impressive signal amplification.
随着 COVID-19 大流行的爆发，生物成像和生物传感的重要性已经显而易见。除了病毒检测外，还需要检测肿瘤、葡萄糖水平和微生物，以改善疾病的治疗和预防。生物纳米粒子，如细胞外囊泡和蛋白质纳米粒子，由于其高表面功能化密度和大容量的负载能力，是生物传感和生物成像应用的理想平台。将大量的传感模块和检测模块支架到生物纳米粒子上，可以提高分析物的亲和力和特异性，并进行信号放大，从而实现即使在低分析物浓度下也具有高度灵敏的检测。在这里，我们通过强调文献中的最新实例，展示了生物纳米粒子在生物成像和生物传感方面的潜力，这些实例利用蛋白质纳米粒子和细胞外囊泡来产生具有令人印象深刻的信号放大的高灵敏度检测装置。
