Badhe Ravindra V, Adkine Deepak, Godse Anagha
Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, Maharashtra, India
Turk J Pharm Sci. 2021 Jun 18;18(3):367-375. doi: 10.4274/tjps.galenos.2020.47897.
This work illustrates a novel method of fabrication of polymeric microneedle (MN) construct using bees wax as mould and development of coated polymeric MNs for drug delivery.
A novel method of MN fabrication using bees wax as mould was established. The porous chitosan MN arrays were fabricated and coated with polylactic acid (PLA). The optimized MN arrays were coated with bovine serum albumin (BSA). The MNs were subjected to physiochemical and tensile strength characterization, followed by drug release study. The skin penetration and irritation study were performed in Wistar Albino rats.
The constructed MN arrays contain MNs with 0.9 mm length, 600 μm width at the base, 30-60 μm diameter at the tip, and 1.5 mm distance between 2 needles. These MNs patch was having good mechanical strength (0.72 N/needle) and tensile strength 15.23 Mpa. The MN array patch had 6.26% swelling index and 98.5% drug release was observed on the 50 hr. Good penetration and no skin irritation was observed for optimized MN batch.
Polymeric MN arrays were successfully developed using bees wax mould and were successfully coated with PLA to deliver the BSA through skin epidermis layer.
本研究阐述了一种以蜂蜡为模具制备聚合物微针（MN）结构的新方法，并开发用于药物递送的包衣聚合物微针。
建立了一种以蜂蜡为模具制备微针的新方法。制备了多孔壳聚糖微针阵列，并用聚乳酸（PLA）进行包衣。对优化后的微针阵列用牛血清白蛋白（BSA）进行包衣。对微针进行理化和拉伸强度表征，随后进行药物释放研究。在Wistar白化大鼠身上进行皮肤渗透和刺激性研究。
构建的微针阵列包含长度为0.9 mm、基部宽度为600 μm、尖端直径为30 - 60 μm且两针间距为1.5 mm的微针。这些微针贴片具有良好的机械强度（0.72 N/针）和拉伸强度15.23 Mpa。微针阵列贴片的溶胀指数为6.26%，在50小时时观察到98.5%的药物释放。优化后的微针批次显示出良好的渗透性且无皮肤刺激性。
利用蜂蜡模具成功开发了聚合物微针阵列，并成功用PLA包衣以通过皮肤表皮层递送BSA。
