Ahlström H, Christofferson R, Lörelius L E
Department of Diagnostic Radiology, Akademiska sjukhuset, Uppsala, Sweden.
APMIS. 1988 Aug;96(8):701-10. doi: 10.1111/j.1699-0463.1988.tb00933.x.
The macro- and microvasculature of the human colonic cancer cell line LS 174 T, 2-8 weeks after subcutaneous deposition in both hind legs of congenitally athymic rats was investigated by light microscopy, angiography, and microvascular corrosion casting with analysis in a scanning electron microscope. The tumour blood vessels were connected to branches of the femoral artery. Only the outer 200-500 micron of the tumour was extensively vascularized, with several concentric, incomplete layers of tortuous vessels, resembling onion skin. Light microscopy revealed necrosis and bleeding in the centre of the tumour, especially in the older tumours, which corresponded well to the central avascularity observed in the casts. There was an increase in venular and capillary density and tortousity towards the tumour in the adjacent muscular fascia. It is concluded that the cell line LS 174 T grows invasively inwards and recruits its vessels from the nude rat host. The overall tumour vascular pattern was unorganized, suggesting limited control of new vessel formation. Extravasations of resin, which were encountered in all cast tumours, can be a rough indicator of enhanced vascular permeability.
通过光学显微镜、血管造影术以及在扫描电子显微镜下分析微血管铸型，对先天性无胸腺大鼠双侧后腿皮下接种人结肠癌细胞系LS 174 T 2 - 8周后的大血管和微血管进行了研究。肿瘤血管与股动脉分支相连。仅肿瘤外层200 - 500微米有丰富的血管分布，有几层同心的、不完整的迂曲血管层，类似洋葱皮。光学显微镜显示肿瘤中心有坏死和出血，尤其是在较老的肿瘤中，这与铸型中观察到的中心无血管区域相符。在邻近的肌筋膜中，朝向肿瘤的小静脉和毛细血管密度及迂曲度增加。得出的结论是，细胞系LS 174 T向内侵袭性生长，并从裸鼠宿主募集血管。肿瘤整体血管模式无组织，提示对新血管形成的控制有限。在所有铸型肿瘤中都发现了树脂外渗，这可能是血管通透性增加的一个粗略指标。
