Khuanekkaphan Monsicha, Khobjai Warachate, Noysang Chanai, Wisidsri Nakuntwalai, Thungmungmee Suradwadee
Department of Health and Aesthetics, Thai Traditional Medicine College, Rajamangala University of Technology, Thanyaburi, Pathum Thani, Thailand.
Department of Clinical Chemistry, Faculty of Medical Technology, Nation University, Lampang, Thailand.
J Adv Pharm Technol Res. 2021 Apr-Jun;12(2):162-168. doi: 10.4103/japtr.JAPTR_254_20. Epub 2021 Apr 27.
The aim of this research was to determine the total phenolic content (TPC), antioxidation, antiaging, and antibacterial activities of Linn., and aims at the novel plant sources which is utilized for their cosmeceutical applications. The two conditions (fresh and dried) and three stages (unripe, ripe, and fully ripe) of were extracted by ethanolic maceration. Folin-Ciocalteu assay was used for determining the TPC. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays were used for estimating antioxidant activity. The inhibitory tyrosinase activities were measured using the modified dopachrome assay. Antiaging was evaluated by inhibition of collagenase and elastase, and antibacterial activities. The result of six extracts from showed that the highest phenolic content and elastase inhibition of the fresh fruit in fully ripe stage were 100.31 ± 2.64 mg GAE/g extract and 14.11% ± 0.95%, respectively. The fresh fruit in the unripe stage showed that the strongest percentage of DPPH IC and collagenase inhibitory activity were 29.11 ± 0.23 μg/mL and 85.94% ± 2.21%, respectively. The ethanolic extract of unripe dried fruit exhibited the highest antioxidant activity in the of ABTS assay, with an IC of 0.17 ± 0.01 μg/mL. The MBC displayed the dried fruit ripe stage anti , and strains were 25.0, 25.0, and 16.25 mg/mL, respectively. The fresh fruit in the ripe stage showed that the strongest inhibition tyrosinase was 93.88% ± 5.64%. The conclusion of this research indicates that the fresh fruit of fruit extracts has high potential as a novel cosmeceuticals' applications to antiaging and skin whitening. The dried fruit in ripe stage extract has the most effective ingredient for antiacne products.
本研究的目的是测定[植物名称]的总酚含量（TPC）、抗氧化、抗老化和抗菌活性，并针对用于其药妆应用的新型植物来源展开研究。[植物名称]的两种状态（新鲜和干燥）以及三个阶段（未成熟、成熟和完全成熟）均通过乙醇浸渍法进行提取。采用福林-酚法测定TPC。使用2,2-二苯基-1-苦基肼（DPPH）和2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）法评估抗氧化活性。采用改良的多巴色素法测定酪氨酸酶抑制活性。通过抑制胶原酶和弹性蛋白酶以及抗菌活性来评估抗老化能力。[植物名称]六种提取物的结果表明，完全成熟阶段新鲜果实的最高酚含量和弹性蛋白酶抑制率分别为100.31±2.64毫克没食子酸当量/克提取物和14.11%±0.95%。未成熟阶段的新鲜果实显示，DPPH IC50的最强百分比和胶原酶抑制活性分别为29.11±0.23微克/毫升和85.94%±2.21%。未成熟干燥果实的乙醇提取物在ABTS法测定中表现出最高的抗氧化活性，IC50为0.17±0.01微克/毫升。MBC显示干燥果实成熟阶段对[具体细菌名称]和[具体细菌名称]菌株的抗菌活性分别为25.0、25.0和16.25毫克/毫升。成熟阶段的新鲜果实显示，最强的酪氨酸酶抑制率为93.88%±5.64%。本研究的结论表明，[植物名称]新鲜果实提取物在抗老化和皮肤美白的新型药妆应用方面具有很高的潜力。成熟阶段干燥果实提取物是抗痤疮产品中最有效的成分。
