Department of Pharmaceutical Sciences, Pharmacognosy and Phytochemistry Division, Rashtrasant Tukadoji Maharaj Nagpur University, Amravati Road, Nagpur 440 033, Maharashtra, India. pri
J Ethnopharmacol. 2011 May 17;135(2):430-3. doi: 10.1016/j.jep.2011.03.036. Epub 2011 Mar 23.
Carissa carandas commonly known as Karanda have a long history of use in traditional system of medicine. It is used by tribal healers of Western Ghat region of Karnataka as hepatoprotective and antihyperglycemic. However, no scientific data is available to validate the folklore claim. The present study has been designed to evaluate its unripe fruit for the antidiabetic activity.
In the present study, methanol extract of unripe fruits and its fractions were studied for its antidiabetic potential.
The methanol extract and its fractions were screened for antidiabetic activity in alloxan induced diabetic rats. The polyphenolic, flavonoid and flavanone contents of methanolic extract and its fractions were also determined and correlated with its antidiabetic activity.
The experimental data indicated that the methanol extract and its ethyl acetate soluble fraction has significantly lowered the elevated blood glucose levels by 48% (p<0.001) and 64.5% (p<0.001) respectively at dose level of 400mg/kg per oral after 24h as compared to diabetic control. In order to assess the role of polyphenolic components in the relevant activity, polyphenolic and flavonoid contents were determined. The polyphenolic and flavonoid content of methanol extract and its ethyl acetate soluble fraction were found to be 15.8 ± 1.2mg and 18.55 ± 0.34 mg (gallic acid equivalent/g extract) and flavonoid content 2.92 ± 0.03 mg and 1.534 ± 0.30 mg (rutin equivalent/g extract) respectively.
The increased antidiabetic potential of ethyl acetate fraction over methanol extract is due to its partial purification achieved by fractionation which resulted in increase in degree of polymerization and segregation of secondary metabolites.
卡拉西卡兰达（Carissa carandas）通常被称为卡兰达，在传统医学体系中已有悠久的使用历史。它被卡纳塔克邦西高止山脉地区的部落治疗师用作保肝和降血糖药。然而，目前尚无科学数据可以验证民间传说的说法。本研究旨在评估其未成熟果实的抗糖尿病活性。
本研究研究了未成熟果实的甲醇提取物及其馏分的抗糖尿病潜力。
在所有oxan诱导的糖尿病大鼠中筛选甲醇提取物及其馏分的抗糖尿病活性。还测定了甲醇提取物及其馏分的多酚、类黄酮和黄烷酮含量，并将其与抗糖尿病活性相关联。
实验数据表明，与糖尿病对照组相比，甲醇提取物及其乙酸乙酯可溶部分在 400mg/kg 口服剂量下，分别在 24 小时后显著降低了升高的血糖水平，分别降低了 48%（p<0.001）和 64.5%（p<0.001）。为了评估多酚成分在相关活性中的作用，测定了多酚和类黄酮含量。甲醇提取物及其乙酸乙酯可溶部分的多酚和类黄酮含量分别为 15.8±1.2mg 和 18.55±0.34mg（没食子酸当量/g 提取物）和类黄酮含量 2.92±0.03mg 和 1.534±0.30mg（芦丁当量/g 提取物）。
与甲醇提取物相比，乙酸乙酯部分的抗糖尿病潜力增加是由于通过分馏实现了部分纯化，导致聚合度增加和次生代谢物的分离。
