Long Non-coding RNA 332443 Inhibits Preadipocyte Differentiation by Targeting Runx1 and p38-MAPK and ERK1/2-MAPK Signaling Pathways.

Long non-coding RNAs (lncRNAs) have emerged as integral regulators of pathophysiological processes, but their specific roles and mechanisms in adipose tissue development remain largely unknown. Here, through microarray analysis, co-expression, and tissue specific analysis of adipocyte tissues after fasting for 72 h, we found that expression was dramatically decreased, as well as the expression of . The UCSC database and Ensembl database indicated that is the antisense lncRNA of . and are highly enriched in adipose tissue and downregulated during adipogenic differentiation. Adipose tissue-specific knockdown of increased fat mass , and specific knockdown of in 3T3-L1 preadipocytes promoted adipogenic differentiation. In this process, expression was decreased when was downregulated in adipocytes or 3T3-L1 preadipocytes, and vice versa, when was upregulated, the expression of was increased. However, overexpression of decreased the expression of the adipocyte cell marker genes γ, α and significantly, while not affected the expression of Mechanistically, positively regulates expression in mouse adipocytes and suppresses adipocyte differentiation by attenuating the phosphorylation of MAPK-p38 and MAPK-ERK1/2 expression. Thus, this study indicated that inhibits adipogenesis and which might be a drug target for the prevention and treatment of obesity.