Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada.
Chem Commun (Camb). 2021 Jul 13;57(56):6871-6874. doi: 10.1039/d1cc02546e.
The trans-cleavage activity of the target-activated CRISPR/Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated the assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and CRISPR/Cas12a resulted in visual detection of the N gene and E gene of SARS-CoV-2 in 45 min.
目标激活的 CRISPR/Cas12a 的跨切割活性从分子转导器中释放出一种 RNA 交联剂，从而促进了金纳米粒子的组装。将分子转导器与等温扩增和 CRISPR/Cas12a 结合，可在 45 分钟内可视化检测 SARS-CoV-2 的 N 基因和 E 基因。
