通过报告基因指导的发酵培养基选择从链霉菌中靶向发现多烯大环内酯六氢沙罗内酯 A。
Targeted Discovery of the Polyene Macrolide Hexacosalactone A from Streptomyces by Reporter-Guided Selection of Fermentation Media.
机构信息
State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, People's Republic of China.
Key Laboratory of Chemical Biology of Ministry of Education, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, People's Republic of China.
出版信息
J Nat Prod. 2021 Jul 23;84(7):1924-1929. doi: 10.1021/acs.jnatprod.1c00144. Epub 2021 Jun 25.
New approaches are still needed to fully explore the biosynthetic potential of microbes. We recently devised a reporter-guided fermentation media screening approach for targeted activation of cryptic gene clusters. Using this approach, we successfully activated the expression of the gene cluster in sp. LZ35 and discovered a novel polyene macrolide hexacosalactone A ().
仍需要新的方法来充分探索微生物的生物合成潜力。我们最近设计了一种报告基因指导的发酵培养基筛选方法，用于靶向激活隐藏的基因簇。使用这种方法，我们成功地激活了 sp. LZ35 中的基因簇的表达，并发现了一种新型多烯大环内酯 hexacosalactone A()。
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