Diabankana Roderic Gilles Claret, Frolov Mikhail, Keremli Saparmyradov, Validov Shamil Zavdatovich, Afordoanyi Daniel Mawuena
Laboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of the Russian Academy of Sciences, 420111 Kazan, Russia.
Tatar Scientific Research Institute of Agricultural Chemistry and Soil Science, FRC Kazan Scientific Center, Russian Academy of Sciences, 420111 Kazan, Russia.
Microorganisms. 2023 Nov 27;11(12):2872. doi: 10.3390/microorganisms11122872.
Microbial biotechnology plays a crucial role in improving industrial processes, particularly in the production of compounds with diverse applications. In this study, we used bioinformatic approaches to analyze the genomic architecture of MGMM6 and identify genes involved in various metabolic pathways that have significant biotechnological potential. Genome mining revealed that MGMM6 consists of a linear chromosome of 6,932,303 bp, with a high G+C content of 73.5%, lacking any plasmid contigs. Among the annotated genes, several are predicted to encode enzymes such as dye peroxidase, aromatic ring-opening dioxygenase, multicopper oxidase, cytochrome P450 monooxygenase, and aromatic ring hydroxylating dioxygenases which are responsible for the biodegradation of numerous endogenous and xenobiotic pollutants. In addition, we identified genes associated with heavy metal resistance, such as arsenic, cadmium, mercury, chromium, tellurium, antimony, and bismuth, suggesting the potential of MGMM6 for environmental remediation purposes. The analysis of secondary metabolites revealed the presence of multiple biosynthesis gene clusters responsible for producing compounds with potent antimicrobial and metal-chelating activities. Furthermore, laboratory tests conducted under controlled conditions demonstrated the effectiveness of MGMM6 in inhibiting phytopathogenic microbes, decolorizing and degrading aromatic triphenylmethane dyes, particularly Blue Brilliant G250, from wastewater by up to 98 ± 0.15%. Overall, the results of our study highlight the promising biotechnological potential of MGMM6.
微生物生物技术在改进工业生产过程中发挥着关键作用，尤其是在生产具有多种用途的化合物方面。在本研究中，我们运用生物信息学方法分析了MGMM6的基因组结构，并鉴定了参与各种具有重要生物技术潜力的代谢途径的基因。基因组挖掘显示，MGMM6由一条6,932,303 bp的线性染色体组成，G+C含量高达73.5%，且不存在任何质粒重叠群。在注释基因中，有几个预计编码诸如染料过氧化物酶、芳环开环双加氧酶、多铜氧化酶、细胞色素P450单加氧酶和芳环羟基化双加氧酶等酶，这些酶负责多种内源性和外源性污染物的生物降解。此外，我们还鉴定了与重金属抗性相关的基因，如砷、镉、汞、铬、碲、锑和铋，这表明MGMM6在环境修复方面具有潜力。次生代谢产物分析表明存在多个生物合成基因簇，这些基因簇负责产生具有强大抗菌和金属螯合活性的化合物。此外，在受控条件下进行的实验室测试表明，MGMM6在抑制植物病原微生物、使废水中的芳香族三苯甲烷染料（特别是亮蓝G250）脱色和降解方面效果显著，脱色和降解率高达98±0.15%。总体而言，我们的研究结果突出了MGMM6具有广阔的生物技术潜力。
