Laboratorio de Biotecnología Ambiental, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.
Unidad Profesional Interdisciplinaria en Ingeniería y Tecnologías Avanzadas, Instituto Politécnico Nacional, Mexico City, Mexico.
Lett Appl Microbiol. 2021 Oct;73(4):446-452. doi: 10.1111/lam.13526. Epub 2021 Jul 9.
Benzo(b)fluoranthene (BbF) is a polycyclic aromatic hydrocarbon (PAH) with five fused benzene rings; it is a highly recalcitrant compound and a priority environmental pollutant due to its detrimental effects on human health and the survival of wild animals. Biodegradation of BbF by microorganisms is an attractive alternative, and few studies have been focused on this issue. In this work, bacteria with the ability to degrade BbF were isolated and selected. The capability of the isolates to tolerate concentrations of 50 and 75 mg l of BbF in liquid medium was evaluated. The selected isolates were identified by the 16S rRNA gene sequencing as belonging to Bacillus, Gordonia, Pseudomonas, Rhodococcus, Ochrobactrum, and Amycolatopsis. All isolates were tolerant and grew at the BbF concentrations tested, some isolates were more competitive than others, and the most prominent was Amycolatopsis sp. Ver12, which removed 47% of BbF, furthermore, with the addition of yeast extract, removed 59% of the compound. In summary, the report shows that Amycolatopsis sp. Ver12 can degrade BbF efficiently and could be considered for bioremediation of BbF-contaminated environments.
苯并（b）荧蒽（BbF）是一种五环稠环芳烃（PAH），具有五个稠合的苯环；它是一种高度难降解的化合物，也是一种优先的环境污染物，因为它对人类健康和野生动物的生存都有不利影响。微生物对 BbF 的生物降解是一种很有吸引力的替代方法，很少有研究关注这个问题。在这项工作中，分离和选择了能够降解 BbF 的细菌。评估了分离物在液体培养基中耐受 50 和 75 mg l BbF 浓度的能力。通过 16S rRNA 基因测序将所选分离物鉴定为属于芽孢杆菌、戈登氏菌、假单胞菌、红球菌、弧菌和拟无枝酸菌。所有分离物都耐受并在测试的 BbF 浓度下生长，一些分离物比其他分离物更具竞争力，其中最突出的是拟无枝酸菌 Ver12，它能去除 47%的 BbF，此外，添加酵母提取物后，能去除 59%的化合物。总之，该报告表明拟无枝酸菌 Ver12 能有效地降解 BbF，可考虑用于 BbF 污染环境的生物修复。
