Yamamoto T, Yokota T
Department of Bacteriology, School of Medicine, Juntendo University, Tokyo, Japan.
Infect Immun. 1988 Oct;56(10):2753-9. doi: 10.1128/iai.56.10.2753-2759.1988.
Vibrio cholerae O1, irrespective of the biotype or serotype, adhered to and was entrapped in the mucus coat covering the mucosal surface of isolated human ileal segments. The evidence for such mucus coat adherence was obtained by treatment of the ileal segments with 10% Formalin. In any case, adherence to the mucus coat was much more prominent than adherence to the epithelial cell surface of the small intestinal villi. Mucus coat adherence was affected by sugars and by the growth phase of the bacterial culture and was diminished by the heating of V. cholerae O1. We conclude that the small intestinal mucus coat is a primary adherence target for V. cholerae O1 in human infection and that the cell-associated hemagglutinin of V. cholerae O1 plays a role, at least in part, in adherence.
霍乱弧菌O1，无论其生物型或血清型如何，都会黏附并被困在覆盖分离出的人回肠段黏膜表面的黏液层中。通过用10%福尔马林处理回肠段获得了这种黏液层黏附的证据。在任何情况下，对黏液层的黏附都比对小肠绒毛上皮细胞表面的黏附更为显著。黏液层黏附受糖类和细菌培养生长阶段的影响，加热霍乱弧菌O1会使其减弱。我们得出结论，小肠黏液层是霍乱弧菌O1在人类感染中的主要黏附靶点，且霍乱弧菌O1的细胞相关血凝素至少在一定程度上参与了黏附过程。
