Nakasone N, Iwanaga M
Department of Bacteriology, University of the Ryukyus School of Medicine, Okinawa.
Microbiol Immunol. 1987;31(8):753-61. doi: 10.1111/j.1348-0421.1987.tb03137.x.
A new method to evaluate the adhesive ability of Vibrio cholerae O1 was proposed. Broth cultured V. cholerae O1 and a piece of formalin-fixed rabbit intestinal wall were incubated together in KRT buffer and the number of adhered organisms was counted under a scanning electron microscope. This method was much less laborious than other methods that have been used so far, and most significantly, constant results were obtained in repeated experiments. The adhesive properties of toxigenic V. cholerae O1 evaluated by this method correlated well with its observed experimental pathogenicity.
提出了一种评估霍乱弧菌O1粘附能力的新方法。将肉汤培养的霍乱弧菌O1与一片福尔马林固定的兔肠壁在KRT缓冲液中共同孵育，然后在扫描电子显微镜下计数粘附的细菌数量。该方法比迄今为止使用的其他方法省力得多，最重要的是，在重复实验中获得了稳定的结果。用该方法评估的产毒霍乱弧菌O1的粘附特性与其观察到的实验致病性密切相关。
