Sincharoenkul R, Chaicumpa W, Pongponratn E, Limpananont J, Tapchaisri P, Kalambaheti T, Chongsa-nguan M
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 1993 Dec;11(2):155-65.
Colonization of V. cholerae O1 in vivo is known to be a non-invasive type which the vibrios are confined only to the intestinal tissues. The pathway by which the vibrio antigens reach the lymphoid cells and subsequently give rise to the immune responses is not entirely clear. Thus, experiments were performed in experimental rats by inoculating live V. cholerae O1 into the ligated ileal loops. The fate of the vibrios in the intestinal tissues was then studied by transmission electron microscopy at different times after the inoculation. It was concluded that live V. cholerae O1 were initially taken up by the M cells which overlay Peyer's patches and which subsequently delivered the intact vibrios to phagocytic cells in the Peyer's patches. These phagocytic cells processed (digested) the vibrios while the lymphocytes and plasma cells infiltrated around them. During the late period of infection (12-15 hours after inoculation of the vibrios), vibrios were also found passing through the loose intercellular spaces between the absorptive epithelial cells into the underlying intestinal tissues.
已知霍乱弧菌O1型在体内的定殖属于非侵袭性类型，即弧菌仅局限于肠道组织。弧菌抗原到达淋巴细胞并随后引发免疫反应的途径尚不完全清楚。因此，通过将活的霍乱弧菌O1接种到结扎的回肠袢中，在实验大鼠身上进行了实验。然后在接种后的不同时间，通过透射电子显微镜研究弧菌在肠道组织中的命运。得出的结论是，活的霍乱弧菌O1最初被覆盖派伊尔结的M细胞摄取，随后M细胞将完整的弧菌递送至派伊尔结中的吞噬细胞。这些吞噬细胞处理（消化）弧菌，同时淋巴细胞和浆细胞在它们周围浸润。在感染后期（接种弧菌后12 - 15小时），还发现弧菌通过吸收性上皮细胞之间的疏松细胞间隙进入下方的肠道组织。
