Wang Jing-Xuan, Wang Bo, Cui Lu-Bin, Xie Hao, Li Run-Zhi, Wang Cheng, Nan Zhang-Jie, Liu Yu-Shu, Ma Jing-Yu, Sun Yun-Jin, Li Wei-Yu
Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Beijing University of Agriculture, Beijing, 102206 China.
Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Detection Control of Spoilage Microorganisms Pesticide Residues in Agricultural Products, Food Science and Engineering College, Beijing University of Agriculture, Beijing, 102206 China.
Physiol Mol Biol Plants. 2021 Jun;27(6):1311-1321. doi: 10.1007/s12298-021-01018-x. Epub 2021 Jun 10.
Aluminum (Al) toxicity is a major limitation to crop production in countries where acidic soil is abundant. In China, soybean production is constrained by Al stress-induced toxicity. As such, there is growing interest to develop Al-resistant varieties. In the present study, we sought to determine potential genes, functions and pathways for screening and breeding of Al-resistant varieties of soybean. First, we mined the E-GEOD-18517 dataset and identified 729 differentially expressed genes (DEGs) between untreated and Al-treated groups. Next, we performed Gene Ontology and Kyoto Encyclopedia of Genes and Genome pathways enrichment analysis and observed that most of the screened genes were mainly enriched in defense response, plasma membrane and molecular transducer activity. They were also enriched in three important pathways, the phenylpropanoid biosynthesis, plant-pathogen interaction, and cutin, suberine and wax biosynthesis. Utilizing weighted gene co-expression network analysis of 815 DEGs screened by Venn diagram, we identified DEGs that were the most disparate between treated and untreated groups. LOC100793667 (probable protein phosphatase 2C 60, GLYMA_17G223800), LOC100780576 (ethylene-responsive transcription factor 1B, GLYMA_02G006200), and LOC100785578 (protein ESKIMO 1, GLYMA_02G258000) were the most differentially expressed, which were consistent with the qRT-PCR results. As these genes are known to participate in essential functions, such as cell junction and phenylpropanoid biosynthesis, these genes may be important for breeding Al-resistant varieties.
The online version contains supplementary material available at 10.1007/s12298-021-01018-x.
铝（Al）毒性是酸性土壤丰富国家作物生产的主要限制因素。在中国，大豆生产受到铝胁迫诱导的毒性限制。因此，人们对培育抗铝品种的兴趣日益浓厚。在本研究中，我们试图确定用于大豆抗铝品种筛选和育种的潜在基因、功能和途径。首先，我们挖掘了E-GEOD-18517数据集，确定了未处理组和铝处理组之间的729个差异表达基因（DEG）。接下来，我们进行了基因本体论和京都基因与基因组百科全书途径富集分析，观察到大多数筛选出的基因主要富集在防御反应、质膜和分子转导活性中。它们还富集在三个重要途径中，即苯丙烷生物合成、植物-病原体相互作用以及角质、木栓质和蜡质生物合成。利用通过维恩图筛选出的815个DEG进行加权基因共表达网络分析，我们确定了处理组和未处理组之间差异最大的DEG。LOC100793667（可能的蛋白磷酸酶2C 60，GLYMA_17G223800）、LOC100780576（乙烯响应转录因子1B，GLYMA_02G006200）和LOC100785578（蛋白ESKIMO 1，GLYMA_02G258000）差异表达最为显著，这与qRT-PCR结果一致。由于已知这些基因参与细胞连接和苯丙烷生物合成等重要功能，这些基因可能对培育抗铝品种很重要。
在线版本包含可在10.1007/s12298-021-01018-x获取的补充材料。
