荧光微管在光照下会分解。
Fluorescent microtubules break up under illumination.
作者信息
Vigers G P, Coue M, McIntosh J R
机构信息
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.
出版信息
J Cell Biol. 1988 Sep;107(3):1011-24. doi: 10.1083/jcb.107.3.1011.
Abstract
We have synthesized three new fluorescent analogues of tubulin, using fluorescein or rhodamine groups attached to N-hydroxy-succinimidyl esters, and have partially characterized the properties of these analogues. We have also further characterized the tubulin derivatized with dichlorotriazinyl-aminofluorescein that has previously been used in this and other laboratories. Our results show that all four analogues assemble into microtubules which break up when exposed to light of the wavelengths that excite fluorescence. This sensitivity places severe constraints on the use of these analogues in studies of microtubule dynamics.
摘要
我们合成了三种新的微管蛋白荧光类似物，使用连接到N-羟基琥珀酰亚胺酯上的荧光素或罗丹明基团，并对这些类似物的性质进行了部分表征。我们还进一步表征了用二氯三嗪基-氨基荧光素衍生化的微管蛋白，该衍生物此前已在本实验室和其他实验室使用。我们的结果表明，所有四种类似物都能组装成微管，当暴露于激发荧光的波长的光下时会分解。这种敏感性对这些类似物在微管动力学研究中的使用施加了严格限制。
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