低/高适应潜力大鼠模型运动的肌因子反应。

Myokine Responses to Exercise in a Rat Model of Low/High Adaptive Potential.

机构信息

Medical Research Council (MRC)-Versus Arthritis Centre for Musculoskeletal Ageing Research, Clinical, Metabolic and Molecular Physiology, University of Nottingham, Derby, United Kingdom.

Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia.

出版信息

Front Endocrinol (Lausanne). 2021 Jun 9;12:645881. doi: 10.3389/fendo.2021.645881. eCollection 2021.

INTRODUCTION

Assuming myokines underlie some of the health benefits of exercise, we hypothesised that 'high responder trainer' (HRT) rats would exhibit distinct myokine profiles to 'low responder trainers' (LRT), reflecting distinct health and adaptive traits.

METHODS

Blood was collected from LRT and HRT (N=8) rats at baseline (BL), immediately (0h), 1h, and 3h after running; repeated after 3-wks training. Myokines were analysed by ELISA (i.e. BDNF/Fractalkine/SPARC/Irisin/FGF21/Musclin/IL-6).

RESULTS

At baseline, Musclin (LRT: 84 ± 24 vs HRT: 26 ± 3 pg/ml, ) and FGF21 (LRT: 133 ± 34 vs HRT: 63.5 ± 13 pg/ml, ) were higher in LRT than HRT. Training increased Musclin in HRT (26 ± 3 to 54 ± 9 pg/ml, ) and decreased FGF21 in LRT (133 ± 34 to 60 ± 28 pg/ml, ). Training increased SPARC (LRT: 0.8 ± 0.1 to 2.1 ± 0.6 ng/ml, ; HRT: 0.7 ± 0.06 to 1.8 ± 0.3 ng/ml, ) and Irisin (LRT 0.62 ± 0.1 to 2.6 ± 0.4 ng/ml, ; HRT 0.53 ± 0.1 to 2.8 ± 0.7 ng/ml, ) while decreasing BDNF (LRT: 2747 ± 293 to 1081 ± 330 pg/ml, ; HRT: 1976 ± 328 to 797 ± 160 pg/ml, ). Acute exercise response of Musclin (AUC) was higher in LRT vs HRT (306 ± 74 vs. 88 ± 12 pg/ml×3h, ) and elevated in HRT after training (221 ± 31 pg/ml×3h, ). Training elevated SPARC (LRT: 2.4 ± 0.1 to 7.7 ± 1.3 ng/ml×3h, ; HRT: 2.5 ± 0.13 to 11.2 ± 2.2 ng/ml×3h, ) and Irisin (LRT: 1.34 ± 0.3 to 9.6 ± 1.7 ng/ml×3h, ; HRT: 1.5 ± 0.5 to 12.1 ± 1.9 ng/ml×3h, ).

CONCLUSION

Exercise training alters how myokines are secreted in response to acute exercise. Myokine responses were not robustly linked to adaptive potential in aerobic capacity, making them an unlikely regulator of adaptive traits.

简介

假设肌肉因子是运动带来部分健康益处的基础，我们推测“高反应训练者”（HRT）大鼠相对于“低反应训练者”（LRT）会表现出明显不同的肌肉因子特征，反映出不同的健康和适应特征。

方法

在基线（BL）、运动后即刻（0h）、1h 和 3h 时，从 LRT 和 HRT（N=8）大鼠中采集血液；运动后 3 周重复采集。使用 ELISA 分析肌肉因子（即 BDNF/Fractalkine/SPARC/Irisin/FGF21/Musclin/IL-6）。

结果

在基线时，LRT 中的 Musclin（84 ± 24 比 HRT：26 ± 3 pg/ml，）和 FGF21（LRT：133 ± 34 比 HRT：63.5 ± 13 pg/ml，）水平高于 HRT。训练增加了 HRT 中的 Musclin（26 ± 3 到 54 ± 9 pg/ml，），降低了 LRT 中的 FGF21（133 ± 34 到 60 ± 28 pg/ml，）。训练增加了 LRT 中的 SPARC（0.8 ± 0.1 到 2.1 ± 0.6 ng/ml，；HRT：0.7 ± 0.06 到 1.8 ± 0.3 ng/ml，）和 Irisin（LRT 0.62 ± 0.1 到 2.6 ± 0.4 ng/ml，；HRT 0.53 ± 0.1 到 2.8 ± 0.7 ng/ml，），同时降低了 BDNF（LRT：2747 ± 293 到 1081 ± 330 pg/ml，；HRT：1976 ± 328 到 797 ± 160 pg/ml，）。LRT 相对于 HRT，肌肉因子 Musclin 的急性运动反应（AUC）更高（306 ± 74 比 88 ± 12 pg/ml×3h，），且 HRT 在训练后更高（221 ± 31 pg/ml×3h，）。训练增加了 LRT 中的 SPARC（2.4 ± 0.1 到 7.7 ± 1.3 ng/ml×3h，；HRT：2.5 ± 0.13 到 11.2 ± 2.2 ng/ml×3h，）和 Irisin（LRT：1.34 ± 0.3 到 9.6 ± 1.7 ng/ml×3h，；HRT：1.5 ± 0.5 到 12.1 ± 1.9 ng/ml×3h，）。