Wang Zheng, Song Hai-Yan, An Meng-Meng, Zhu Li-Li
Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Harbin Medical University, Hormone and Endocrinology Key Laboratory of Harbin Medical University Xuefu Road 246, Harbin 150080, China.
Int J Clin Exp Med. 2015 Oct 15;8(10):19290-6. eCollection 2015.
To investigate the association of serum SPARC level with the severity of coronary artery lesion in type 2 diabetic patients with coronary heart disease.
120 patients with type 2 diabetic patients were the subjects. Enzyme-linked immunosorbent assay (ELISA) was used to detect levels of serum SPARC and Gensini score was used to assess extent of coronary artery lesions. The patients were divided into 4 groups: A group was the healthy control group with 40 patients. According to angiography and the World Health Organization (WHO) diagnostic criteria for diabetes the rest were divided into B, C, D group: there were 40 cases in group B (simple type 2 diabetes mellitus group), 40 cases were in group C (simple CHD group), and 40 cases were in D group (type 2 diabetes combined with coronary heart disease group).
Compared with that in group A, the serum SPARC level in group B, C and D increased significantly (4.22±1.19) μg/L, (3.71±1.05) μg/L and (5.96±1.40) μg/L vs (3.60±0.40) μg/L (P<0.05 ). Moreover, the serum SPARC level in group D was the highest (P<0.05). Serum SPARC level, insulin resistance (IR), and glycosylated hemoglobin (HbA1c) were the vital factors contributing to coronary heart disease. Serum SPARC level was positively correlated with the Gensini scores in group D (r=0.770, P<0.05), whereas it was not related to the Gensini scores in group C (r=0.520, P>0.05). Pearson correlation analysis showed that serum SPARC level was positively correlated with triglyceride, fasting insulin, Homeostasis Model Assessment for Insulin Resistance Index (r=0.780, 0.762 and 0.891, respectively; P<0.05).
Serum SPARC level elevated in T2DM patients with coronary heart disease, which was correlated with the severity of coronary artery disease significantly.
探讨2型糖尿病合并冠心病患者血清富含半胱氨酸的酸性分泌蛋白（SPARC）水平与冠状动脉病变严重程度的关系。
选取120例2型糖尿病患者作为研究对象。采用酶联免疫吸附测定（ELISA）法检测血清SPARC水平，采用Gensini评分评估冠状动脉病变程度。将患者分为4组：A组为健康对照组，40例。其余患者根据血管造影及世界卫生组织（WHO）糖尿病诊断标准分为B、C、D组：B组（单纯2型糖尿病组）40例，C组（单纯冠心病组）40例，D组（2型糖尿病合并冠心病组）40例。
与A组相比，B、C、D组血清SPARC水平显著升高（分别为4.22±1.19μg/L、3.71±1.05μg/L和5.96±1.40μg/L， vs 3.60±0.40μg/L，P<0.05）。此外，D组血清SPARC水平最高（P<0.05）。血清SPARC水平、胰岛素抵抗（IR）和糖化血红蛋白（HbA1c）是导致冠心病的重要因素。D组血清SPARC水平与Gensini评分呈正相关（r=0.770，P<0.05），而C组血清SPARC水平与Gensini评分无关（r=0.520，P>0.05）。Pearson相关性分析显示，血清SPARC水平与甘油三酯、空腹胰岛素、胰岛素抵抗稳态模型评估指数呈正相关（r分别为0.780、0.762和0.891；P<0.05）。
2型糖尿病合并冠心病患者血清SPARC水平升高，且与冠状动脉疾病严重程度显著相关。
