Woods G L, Yamamoto M, Young A
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68105.
J Virol Methods. 1988 Jun;20(2):109-14. doi: 10.1016/0166-0934(88)90144-9.
Two methods for rapid detection of adenovirus were tested: (i) 24-well plate centrifugation followed by staining with a monoclonal antibody after incubation for 24 h and 48 h, and (ii) pretreatment of A549 cells used in conventional cell culture and 24-well plate centrifugation with 10(-5)M dexamethasone. Twenty-seven clinical isolates of adenovirus and 12 specimens from which adenovirus had been recovered were included in the analysis. Both isolates and specimens had been frozen at -70 degrees C for up to 6 months. By 24-well plate centrifugation both with and without dexamethasone, 21 (78%) and 27 (100%) isolates were positive for adenovirus at 24 h and 48 h, respectively. Of the specimens, 6 (50%) and 8 (67%) were positive by 24-well plate centrifugation without dexamethasone at 24 h and 48 h, respectively, whereas with dexamethasone 3 (25%) were positive at 24 h and 7 (58%) were positive at 48 h. Overall, combining isolates and specimens, the sensitivity of 24-well plate centrifugation for detection of adenovirus at 24 h was 69% without dexamethasone and 62% with dexamethasone, and at 48 h the sensitivity was 90% without dexamethasone and 87% with dexamethasone. The specificity under all conditions tested was 100%. In conventional tissue culture dexamethasone inhibited recovery of adenovirus. Without dexamethasone, adenovirus was recovered from all 39 samples within 7 days after inoculation; however with dexamethasone pretreatment, the virus was detected in only 31 (79%) of the samples tested in the same period of time.
（i）24孔板离心，在孵育24小时和48小时后用单克隆抗体染色；（ii）用10（-5）M地塞米松对常规细胞培养和24孔板离心所用的A549细胞进行预处理。分析纳入了27株腺病毒临床分离株和12份已从中分离出腺病毒的标本。分离株和标本均已在-70℃下冷冻长达6个月。通过24孔板离心，无论有无地塞米松，分别有21株（78%）和27株（100%）分离株在24小时和48小时时腺病毒检测呈阳性。在标本中，分别有6份（50%）和8份（67%）在24小时和48小时时通过无地塞米松的24孔板离心检测呈阳性，而使用地塞米松时，24小时时有3份（25%）呈阳性，48小时时有7份（58%）呈阳性。总体而言，将分离株和标本合并计算，无地塞米松时24孔板离心检测腺病毒在24小时时的灵敏度为69%，使用地塞米松时为62%，48小时时无地塞米松时灵敏度为90%，使用地塞米松时为87%。在所有测试条件下特异性均为100%。在常规组织培养中，地塞米松抑制腺病毒的回收。无地塞米松时，接种后7天内从所有39个样本中均回收了腺病毒；然而，经过地塞米松预处理后，在同一时间段内仅在31份（79%）测试样本中检测到该病毒。
