Espy M J, Hierholzer J C, Smith T F
Am J Clin Pathol. 1987 Sep;88(3):358-60. doi: 10.1093/ajcp/88.3.358.
Monoclonal antibody, specific for the adenovirus group-reactive hexon antigen, was used for the detection of this agent by immunofluorescence 24 and 48 hours after inoculation of HEp-2 cell monolayers in 1-dram shell vials after low-speed centrifugation (700 X g, 30 minutes). Of 31 adenovirus isolates, 16 (sensitivity, 52%) and 30 (sensitivity, 97%) were detected after incubation for 24 hours and 48 hours, respectively. All adenovirus strains were detected in conventional tube cell cultures but required an average of four days incubation. The shell vial assay is rapid, sensitive, and specific and can be easily implemented in laboratories with cell culture experience.
针对腺病毒群反应性六邻体抗原的单克隆抗体，用于在1 dram（约30毫升）带盖小瓶中接种HEp - 2细胞单层并低速离心（700×g，30分钟）后，通过免疫荧光在接种后24小时和48小时检测该病原体。在31株腺病毒分离株中，分别在孵育24小时和48小时后检测到16株（敏感性52%）和30株（敏感性97%）。所有腺病毒株在传统的试管细胞培养中均能检测到，但平均需要四天的孵育时间。小瓶培养法快速、灵敏且特异，有细胞培养经验的实验室均可轻松实施。
