Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, P.R. China.
College of Life Science, Yangtze University, Jingzhou, 434023, Hubei, P.R. China.
Curr Microbiol. 2021 Aug;78(8):3292-3298. doi: 10.1007/s00284-021-02551-4. Epub 2021 Jun 28.
A Gram-staining-positive, aerobic, non-endospore-forming, coccus-shaped, non-flagellated, and non-motile bacterium, designated WN024, was isolated from the natural saline-alkali wetland soil of Binhai new district, Tianjin, China. Cells of strain WN024 were catalase- and oxidase-positive. The isolate was able to grow between 20 and 45 °C (optimal at 33-37 °C), pH 6.5-11.0 (optimal 7.5-8.0), and in the presence of 5.0-25.0% NaCl (optimal at 10.0-15.0%, w/v). The isolate could be affiliated to the genus Salimicrobium and the highest 16S rRNA gene sequence similarity of strain WN024 to its closest relative Salimicrobium salexigens DSM 22782 was 97.9%. The size of the genome was 2,622,223 bp in size with a G + C content of 47.1%. The sole respiratory quinone of strain WN024 was MK-7, the predominant fatty acids were iso-C, anteiso-C and anteiso-C. The major polar lipids were phosphatidylglycerol (PG), glycolipid (GL), phospholipid (PL) and diphosphatidylglycerol (DPG). The cell-wall diamino acid was meso-diaminopimelic acid. The DNA-DNA hybridization values between strain WN024 and the closest relative S. salexigens DSM 22782 was 47.3 ± 2.3%. The highest average nucleotide identity (ANI) value was 92.3% to S. salexigens DSM 22782 (GenBank Accession No. GCA_900156705.1). Therefore, we propose a novel species in the genus Salimicrobium to accommodate this novel isolate, named Salimicrobium humidisoli sp. nov. The type strain is WN024 (= ACCC 19979 = KCTC 33897).
一株革兰氏阳性、需氧、不产芽孢、球状、无鞭毛、不运动的细菌，被命名为 WN024，从中国天津滨海新区的天然盐碱性湿地土壤中分离得到。WN024 菌株的细胞呈过氧化氢酶和氧化酶阳性。该分离株能够在 20-45°C（最佳 33-37°C）、pH6.5-11.0（最佳 7.5-8.0）和 5.0-25.0%NaCl（最佳 10.0-15.0%，w/v）的条件下生长。该分离株可归属于盐单胞菌属，与最接近的 Salimicrobium salexigens DSM 22782 的 16S rRNA 基因序列相似性最高为 97.9%。WN024 菌株的基因组大小为 2622223bp，G+C 含量为 47.1%。该菌株的唯一呼吸醌为 MK-7，主要脂肪酸为 iso-C、anteiso-C 和 anteiso-C。主要极性脂为磷脂酰甘油（PG）、糖脂（GL）、磷脂（PL）和双磷脂酰甘油（DPG）。细胞壁二氨基庚二酸为 meso-二氨基庚二酸。WN024 菌株与最接近的 Salimicrobium salexigens DSM 22782 的 DNA-DNA 杂交值为 47.3±2.3%。最高的平均核苷酸同一性（ANI）值为 92.3%，与 Salimicrobium salexigens DSM 22782（GenBank 注册号 GCA_900156705.1）最为接近。因此，我们建议将这个新分离株归属于盐单胞菌属中的一个新种，命名为 Salimicrobium humidisoli sp. nov. 。模式菌株为 WN024（=ACCC 19979=KCTC 33897）。
