State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Heilongjiang Academy of Black Soil Conservation & Utilization/Key Lab of Soil Environment and Plant Nutrition of Heilongjiang Province/Heilongjiang Fertilizer Engineering Research Center, Harbin, 150086, People's Republic of China.
Curr Microbiol. 2022 Aug 24;79(10):301. doi: 10.1007/s00284-022-02997-0.
A novel bacterium, designated strain APA_H-1(4), was isolated from the saline-alkaline soil, Zhaodong, Heilongjiang Province, China. Phenotypic and chemotaxonomic analyses, and whole-genome sequencing were used to determine the taxonomic position of the strain. Phylogenetic analysis indicated that the isolate belongs to the genus Oceanobacillus, and showed the highest sequence similarity to O. damuensis KCTC 33146 (98.35%, similarity) and 'O. massiliensis' DSM 24644 (98.32%). The average nucleotide identity values between strain APA_H-1(4) and other members of the genus Oceanobacillus were lower than 82% recommended for distinguishing novel prokaryotic species. The digital DNA-DNA hybridization values of strain APA_H-1(4) with O. damuensis KCTC 33146 and 'O. massiliensis' DSM 24644 were 13.60 and 17.60%, respectively. Cells of strain APA_H-1(4) were Gram-staining positive, motile, aerobic, spore-forming rods (0.5-0.7 × 1.8-2.6 μm) with flagella. The growth was found to occur optimally at 37 °C. The whole-cell hydrolysate contained meso-diaminopimelic acid as the diagnostic cell wall diamino acid. The main detected polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified polar lipid. The predominant respiratory quinone was identified as menaquinone-7 (MK-7). The major cellular fatty acid (>10%) was anteiso-C. The G + C content of the genomic DNA was determined to be 38.4% based on the draft genome sequence. Based on the comparative analysis of polyphasic taxonomic data, strain APA_H-1(4) represents a novel species of the genus Oceanobacillus, for which the name Oceanobacillus saliphilus sp. nov. is proposed. The type strain is APA_H-1(4) (=GDMCC 1.2239 = KCTC 43254).
一株新的海洋杆菌，命名为 APA_H-1(4) 菌株，从中国黑龙江肇东的盐碱土壤中分离得到。通过表型和化学分类分析以及全基因组测序来确定该菌株的分类地位。系统发育分析表明，该分离株属于海洋杆菌属，与 O. damuensis KCTC 33146（98.35%，相似度）和“O. massiliensis”DSM 24644（98.32%）的序列相似度最高。与其他海洋杆菌属成员的平均核苷酸同一性值均低于 82%，这是区分新型原核生物种的推荐值。APA_H-1(4) 菌株与 O. damuensis KCTC 33146 和“O. massiliensis”DSM 24644 的数字 DNA-DNA 杂交值分别为 13.60 和 17.60%。APA_H-1(4) 菌株的细胞革兰氏染色阳性，运动，需氧，形成芽孢的杆状（0.5-0.7μm×1.8-2.6μm），有鞭毛。最佳生长温度为 37°C。全细胞水解物含有二氨基庚二酸作为诊断细胞壁二氨基庚二酸。主要检测到的极性脂类由双磷脂酰甘油、磷脂酰甘油、一种未鉴定的磷脂和一种未鉴定的极性脂类组成。主要的呼吸醌为menaquinone-7（MK-7）。主要细胞脂肪酸（>10%）为 anteiso-C。根据基因组 DNA 的草图序列，基因组 DNA 的 G+C 含量确定为 38.4%。基于多相分类数据的比较分析，APA_H-1(4) 菌株代表海洋杆菌属的一个新种，命名为海洋盐杆菌新种。模式菌株为 APA_H-1(4)（=GDMCC 1.2239=KCTC 43254）。
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