Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Jinnan District, Yaguan Road 135, Tianjin, 300350, People's Republic of China.
Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
Arch Microbiol. 2021 Jul;203(5):2211-2217. doi: 10.1007/s00203-021-02239-0. Epub 2021 Feb 24.
A Gram-staining-positive, endospore-forming, aerobic strain, designed WN066, was isolated from saline-alkali wetland soil of Tianjin, China. Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066 was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600 (98.9%), Bacillus cucumis CCM 8651 (98.8%), Bacillus bataviensis DSM 15601 (98.7%) and Bacillus niacini DSM 2923 (98.7%). However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066 and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species. The strain grew at 19-42 °C (optimally 33-37 °C) in the presence of 3-20% (w/v) NaCl (optimally 8-12%(w/v)), and at pH 6.5-11.0 (optimally 7.5-8.5). The major cellular fatty acids were iso-C (24.8%) and anteiso-C (38.9%). The predominant polar lipids consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066 represented a novel species within the genus Bacillus, for which the name B. salipaludis sp. nov. is proposed. The type strain is WN066 (= KCTC 33953 = ACCC 60085).
一株革兰氏阳性、产芽孢、需氧的菌株，设计为 WN066，从中国天津盐碱性湿地土壤中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，WN066 是芽孢杆菌属的一个成员，与德雷顿氏芽孢杆菌 DSM 15600（98.9%）、黄瓜芽孢杆菌 CCM 8651（98.8%）、巴氏芽孢杆菌 DSM 15601（98.7%）和烟酸芽孢杆菌 DSM 2923（98.7%）最为密切相关。然而，菌株 WN066 与最密切相关的种之间的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（DDH）值小于先前提出的区分属内种的界限值，表明该菌株代表了一个新的芽孢杆菌种。该菌株在 19-42°C（最佳 33-37°C）、3-20%（w/v）NaCl（最佳 8-12%（w/v））和 pH 6.5-11.0（最佳 7.5-8.5）的条件下生长。主要细胞脂肪酸为异-C（24.8%）和 anteiso-C（38.9%）。主要极性脂由双磷脂酰甘油（DPG）、磷脂酰甘油（PG）和磷脂酰乙醇胺（PE）组成。基因组大小为 6213503bp，G+C 含量为 38.6mol%。肽聚糖含有 meso-二氨基庚二酸作为诊断二氨基酸。基于表型、系统发育、化学分类和基因组特征，菌株 WN066 代表芽孢杆菌属中的一个新种，命名为巴氏盐沼地芽孢杆菌 sp. nov.。模式菌株为 WN066（=KCTC 33953=ACCC 60085）。
