Department of Marine Chemistry, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, China.
Zhoushan Natural Resources Surveying and Mapping Design Center, Zhoushan, China.
Curr Microbiol. 2021 Nov;78(11):3996-4003. doi: 10.1007/s00284-021-02645-z. Epub 2021 Sep 14.
Marine phycosphere hosts cross-kingdom algae-bacteria interactions playing a variety of crucial roles in aquatic ecosystems especially for the prevention and control of harmful algal blooms (HABs). During the investigation of structural composition of phycosphere microbiota (PM) of diverse marine HAB dinoflagellates, a Gram-negative, strictly aerobic, non-motile and rod-shaped bacterium designated LZ-17 was isolated from the phycosphere of highly toxic Alexandrium catenella LZT09. The 16S rRNA gene sequence analysis and the multilocus sequence analysis (MLSA) based on five protein-coding housekeeping genes (atpD, gyrB, mutL, topA and rpoD) indicated that strain LZ-17 was affiliated to the genus Maritimibacter within the family Rhodobacteraceae, and closely related to Maritimibacter alkaliphilus HTCC2654 (99.1%), 'Maritimibacter harenae' DP07 (97.9%) and M. lacisalsi X12M-4 (95.7%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain LZ-17 and the type strain of M. alkaliphilus were 96.9% and 74.7%. However, strain LZ-17 could be clearly distinguished from its closest by the phenotypical and phenotypical characteristics. Strain LZ-17 contained Q-10 as its major isoprenoid quinone, and summed feature 8 (C ω7c and/or C ω6c), C and C 2-OH as the predominant fatty acids (>10%). The major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. The DNA G + C content was 64.3 mol%. Based on the polyphasic taxonomic characterization, strain LZ-17 represents a novel species of the genus Maritimibacter, for which the name Maritimibacter alexandrii sp. nov. is proposed, with the type strain LZ-17 (=CCTCC 2019005 = KCTC 72193).
海洋菌席宿主跨王国藻类-细菌相互作用在水生生态系统中发挥着多种关键作用，特别是对有害藻华（HAB）的预防和控制。在研究不同海洋赤潮甲藻的菌席微生物组（PM）的结构组成时，从剧毒亚历山大藻 LZT09 的菌席中分离到一株革兰氏阴性、严格需氧、非运动和杆状细菌，命名为 LZ-17。16S rRNA 基因序列分析和基于五个蛋白编码管家基因（atpD、gyrB、mutL、topA 和 rpoD）的多位点序列分析（MLSA）表明，菌株 LZ-17 属于红杆菌科海洋杆菌属，与海洋杆菌 Maritimibacter alkaliphilus HTCC2654（99.1%）、'Maritimibacter harenae' DP07（97.9%）和 M. lacisalsi X12M-4（95.7%）密切相关。菌株 LZ-17 与模式菌株 M. alkaliphilus 的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 96.9%和 74.7%。然而，菌株 LZ-17 可以通过表型和表型特征与最接近的菌株明显区分开来。菌株 LZ-17 含有 Q-10 作为其主要的类异戊二烯醌，和特征脂肪酸总和 8（C ω7c 和/或 C ω6c）、C 和 C 2-OH 作为主要脂肪酸（>10%）。主要极性脂由双磷脂酰甘油、磷脂酰甘油和磷脂酰胆碱组成。DNA G+C 含量为 64.3 mol%。基于多相分类学特征，菌株 LZ-17 代表海洋杆菌属的一个新种，命名为亚历山大海洋杆菌 Maritimibacter alexandrii sp. nov.，其模式菌株为 LZ-17（=CCTCC 2019005=KCTC 72193）。
