Schenk E A, Waag R W, Schenk A B, Aubuchon J P
Department of Pathology, University of Rochester Medical Center, New York 14642.
J Histochem Cytochem. 1988 Oct;36(10):1341-51. doi: 10.1177/36.10.3418109.
We used a scanning acoustic microscope to image normal and outdated red blood cells, cells with different hemoglobin content, red cell ghosts, and cells treated with various drugs that induce echinocyte-stomatocyte transformation. Images were obtained at different planes of focus within the cells, corresponding to maxima and minima of signal intensity. Digitization and gray scale amplitude mapping were used to create axonometric plots that display signal amplitude variations within the cells. The images of red cells contain features produced by differences in topology, density, elasticity, and absorption. Both hemoglobin content and the cell cytoskeleton contribute to image features, and various deformations, characterized by the formation of blebs and vacuoles, are displayed in cells undergoing echinocyte-stomatocyte transformation. These preliminary findings, although mainly descriptive, indicate that acoustic microscopy may be a useful new method for evaluating red cell deformation and associated changes in mechanical properties.
我们使用扫描声学显微镜对正常和陈旧的红细胞、具有不同血红蛋白含量的细胞、红细胞影以及用各种诱导棘红细胞-口形红细胞转化的药物处理过的细胞进行成像。在细胞内不同的聚焦平面获取图像，这些平面对应信号强度的最大值和最小值。通过数字化和灰度幅度映射来创建轴测图，以显示细胞内的信号幅度变化。红细胞图像包含由拓扑结构、密度、弹性和吸收差异产生的特征。血红蛋白含量和细胞骨架都对图像特征有贡献，并且在经历棘红细胞-口形红细胞转化的细胞中会显示出以泡状和液泡形成为特征的各种变形。这些初步发现虽然主要是描述性的，但表明声学显微镜可能是评估红细胞变形及相关力学性质变化的一种有用的新方法。
