Zhou W, Zeng G, Lyu C, Kou F, Zhang S, Wei H
1Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
4Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Physiol Int. 2021 Jun 25;108(2):285-302. doi: 10.1556/2060.2021.00150.
Limited investigations on metabolic responses to exercise training in female adolescent volleyball athletes exist. The aim of this study was to obtain serum and urine metabolite markers in female adolescent volleyball athletes within 2-week strength-endurance training using a metabolomics approach coupled with biochemical analysis, which would be potential biomarkers for evaluating the physiological state of athletes.
Twelve female adolescent volleyball athletes were recruited for 2-week strength-endurance training. Differential serum and urine metabolic profiles between the pre- and post-training group were obtained on gas chromatography coupled to mass spectrometry (GC-MS) and data subsequently underwent orthogonal partial least-squares analysis (OPLS).
Strength-endurance training exerted a significant influence on the athletes' serum and urine metabolic profiles. The changed metabolites were primarily involved in energy metabolism, lipid metabolism and amino acids metabolism. Results support the hypothesis that female athletes displayed an increased propensity to oxidize lipids as the major energy source. Exposure to strength-endurance training also led to a significant increase in cortisol, but a decrease in testosterone, indicating disordered hormone adjustment. Exercise-induced oxidative stress occurred, as was evidenced by the decrease in reduced glutathione, and increases in blood malondialdehyde and oxidized glutathione. Since the muscle damage markers creatine kinase and lactate dehydrogenase did not show significant changes, the training might not cause cell membrane damage and the athletes did not cross the adaptive injury level.
By measurement of endogenous metabolites, the metabolomics study has the potential to reveal the global physiological changes in response to exercise training.
关于女性青少年排球运动员运动训练代谢反应的研究有限。本研究旨在采用代谢组学方法结合生化分析，获取女性青少年排球运动员在为期2周的力量耐力训练期间的血清和尿液代谢物标志物，这些标志物可能成为评估运动员生理状态的生物标志物。
招募12名女性青少年排球运动员进行为期2周的力量耐力训练。通过气相色谱-质谱联用（GC-MS）获得训练前和训练后组之间血清和尿液的差异代谢谱，随后对数据进行正交偏最小二乘法分析（OPLS）。
力量耐力训练对运动员的血清和尿液代谢谱产生了显著影响。变化的代谢物主要涉及能量代谢、脂质代谢和氨基酸代谢。结果支持这样的假设，即女性运动员氧化脂质作为主要能量来源的倾向增加。进行力量耐力训练还导致皮质醇显著增加，但睾酮减少，表明激素调节紊乱。运动诱导的氧化应激发生，这通过还原型谷胱甘肽减少、血液丙二醛和氧化型谷胱甘肽增加得到证明。由于肌肉损伤标志物肌酸激酶和乳酸脱氢酶没有显示出显著变化，训练可能没有导致细胞膜损伤，运动员也没有超过适应性损伤水平。
通过测量内源性代谢物，代谢组学研究有可能揭示运动训练引起的整体生理变化。
