Taranto Francesca, Mangini Giacomo, Miazzi Monica Marilena, Stevanato Piergiorgio, De Vita Pasquale
National Research Council (CNR), Institute of Biosciences and Bioresources (CNR-IBBR), 80055 Portici, NA, Italy.
National Research Council (CNR), Institute of Biosciences and Bioresources (CNR-IBBR), 70126 Bari, BA, Italy.
Genomics. 2021 Sep;113(5):2989-3001. doi: 10.1016/j.ygeno.2021.06.030. Epub 2021 Jun 26.
Studying and understanding the genetic basis of polyphenol oxidases (PPO)-related traits plays a crucial role in genetic improvement of crops. A tetraploid wheat collection (T. turgidum ssp., TWC) was analyzed using the 90K wheat SNP iSelect assay and phenotyped for PPO activity. A total of 21,347 polymorphic SNPs were used to perform genome-wide association analysis (GWA) in TWC and durum wheat sub-groups, detecting 23 and 85 marker-trait associations (MTA). In addition, candidate genes responsible for PPO activity were predicted. Based on the 23 MTAs detected in TWC, two haplotypes associated with low and high PPO activity were identified. Four SNPs were developed and validated providing one reliable marker (IWB75732) for marker assisted selection. The 23 MTAs were used to evaluate the genetic divergence (F > 0.25) between the T. turgidum subspecies, providing new information important for understanding the domestication process of Triticum turgidum ssp. and in particular of ssp. carthlicum.
研究和理解多酚氧化酶（PPO）相关性状的遗传基础在作物遗传改良中起着至关重要的作用。利用90K小麦SNP iSelect分析方法对四倍体小麦群体（T. turgidum ssp.，TWC）进行了分析，并对PPO活性进行了表型鉴定。共使用21,347个多态性SNP在TWC和硬粒小麦亚组中进行全基因组关联分析（GWA），检测到23个和85个标记-性状关联（MTA）。此外，还预测了负责PPO活性的候选基因。基于在TWC中检测到的23个MTA，鉴定出了与低PPO活性和高PPO活性相关的两种单倍型。开发并验证了4个SNP，提供了一个用于标记辅助选择的可靠标记（IWB75732）。利用这23个MTA评估了T. turgidum亚种之间的遗传分化（F>0.25），为理解普通小麦亚种尤其是硬粒小麦亚种的驯化过程提供了重要的新信息。
